2010 Fiscal Year Final Research Report

Research of pathogenesis and treatment of the necrotizing enterocolitis model induced by in vivo real-time two-photon microscopic imaging

Research Project

Project/Area Number	21890107
Research Category	Grant-in-Aid for Research Activity Start-up
Allocation Type	Single-year Grants
Research Field	Pediatric surgery
Research Institution	Mie University
Principal Investigator	KOIKE Yuhki Mie University, 大学院・医学系研究科, リサーチアソシエイト (10555551)
Project Period (FY)	2009 – 2010
Keywords	two-photon laser-scanning microscopy / green fluorescent protein / thrombus formation / laser irradiation / vascular endothelium injury / necrotizing enterocolitis model
Research Abstract	This research project is making the necrotizing enterocolitis model created in beta-actin-green fluorescent protein transgenic mice observed by two-photon laser-scanning microscopy (TPLSM), and figuring out the pathogenesis and treatment of this disease using this model and system. Currently, because the further development of organ stabilizing system identify each of the layers of vascular smooth muscle and endothelium, endothelial layer were identified by selective in vivo staining with fluorescent Griffonia simplicifolia 1134 isolectin. As for the necrotizing enterocolitis model, we invented the new stabilizing system that enable us to observe the mucosal layer of the small intestine without laparotomy in neonatal mice. Now we try to evaluate the real-time change of the mucosal layer and drug efficiency of the necrotizing enterocolitis model induced by LPS injection.