Study of hydrophilicity of DNA-labeled microbeads and development of electric power less visual nucleic acid amplification detection method

2023 Fiscal Year Final Research Report

• Project/Area Number: 21H01731
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 27040:Biofunction and bioprocess engineering-related
• Research Institution: Kyushu University
• Principal Investigator: Nakano Michihiko 九州大学, システム情報科学研究院, 准教授 (00447856)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: 微粒子 / DNA検出 / 迅速検査 / 目視判定 / 診断技術

Outline of Final Research Achievements

It has been developed that direct eye detection of the amplified DNA makes nucleic acid amplification testing rapid and simple. In the project, the principle of the detection method was tried to be revealed. Also, the method was improved to detect multiple samples amplified by multiplex polymerase chain reaction. In the view of the rapid diagnosis techniques, the following subjects have been studied: detection of DNase I, which is a candidate of a myocardial infarction biomarker, using the microbeads dielectrophoresis-based DNA detection method; dielectric characterization of exosomes, which are kinds of extracellular vesicles; dielectric characterization of the malaria-infected erythrocyte. The demonstrated results show the potential of the methods to be rapid and simple diagnosis methods for infectious diseases and cancer.

Free Research Field

静電気生物応用

Academic Significance and Societal Importance of the Research Achievements

DNA結合微粒子によるDNAの目視判定手法について，その原理の一端を明らかにした．これは，これまでに知られていないDNAの物理的性質であり，さらに詳細に調べることでDNAが持つ新たな性質が明らかになる可能性がある．他方，感染症やがん診断を迅速・簡便化するための基礎的な研究として，本研究の手法を応用した．これらの成果は，医療リソースに乏しい地域での感染症検査や，家畜や農作物に対する感染症検査には，簡便で迅速かつ安価な手法はもちろんのこと，その場で検出できる手法を提供する．感染症の脅威は常に私たちのそばにある．本研究は，我々が感染症と戦うために必要不可欠な新しい武器となると期待している．