Ultra-wide field 3D fluorescence imaging for all cell analysis in cultured cell sheets

2023 Fiscal Year Final Research Report

• Project/Area Number: 21H01843
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 30020:Optical engineering and photon science-related
• Research Institution: Osaka University
• Principal Investigator: Taro Ichimura 大阪大学, 先導的学際研究機構, 特任准教授(常勤) (50600748)
• Co-Investigator(Kenkyū-buntansha): 中村 友哉 大阪大学, 産業科学研究所, 准教授 (70756709)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: バイオイメージング / 蛍光顕微鏡 / 培養細胞シート

Outline of Final Research Achievements

In recent years, regenerative medicine using iPS cells has made progress, but our understanding of the process of tissue formation is insufficient, partly due to a lack of imaging technology. To address this issue, this study was undertaken to develop a new imaging method that enables three-dimensional observation of all cells in a cell sheet. Specifically, we proposed a oblique incidence light-sheet fluorescence microscope that combines a wide-field imaging method and a computer-generated hologram technique, established a technique to selectively excite specific planes in a sample, and demonstrated its potential for life science and regenerative medicine.

Free Research Field

バイオイメージング

Academic Significance and Societal Importance of the Research Achievements

細胞シートのように薄くて広い組織の三次元イメージングに適した蛍光イメージング技術を開発し、生命科学者に提供することで、細胞シートの形成メカニズムの理解を進め、高効率な細胞シート作成の技術革新を誘導できる。また、細胞生物学やシステム生物学など、生物学の複数の領域において、多細胞システムと単細胞間の関係を、スケール階層を跨いで理解するための研究スキームを提供することが期待できる。