Development of a non-invasive, rapid, and ultra-sensitive extracellular vesicles detection method for the early detection of ovarian cancer using tears

2023 Fiscal Year Final Research Report

• Project/Area Number: 21H01963
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 34020:Analytical chemistry-related
• Research Institution: Kobe University
• Principal Investigator: Takano Eri 神戸大学, 工学研究科, 学術研究員 (20634645)
• Co-Investigator(Kenkyū-buntansha): 國久 智成 神戸大学, 医学部附属病院, 特命講師 (50839800) ; 寺井 義人 神戸大学, 医学研究科, 特命教授 (90278531)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: EVsセンシングチップ / 卵巣がん / 細胞外小胞 / 涙液 / 自動分析

Outline of Final Research Achievements

In this study, a non-invasive, rapid, and ultra-sensitive detection method for extracellular vesicles (EVs), which have garnered attention for their association with cancer, is developed for the early detection of ovarian cancer. EVs are utilized as a tumor marker.
Using the CD9 antibody-immobilized substrate, purified EVs from cell culture supernatant of human ovarian cancer-derived cell line were measured. The fluorescence intensity changed as the concentration increases, suggesting that the fabricated sensing chip can be used for rapid EVs measurement. The difference in adsorption behavior to different antibodies suggested the possibility of identifying ovarian cancer-derived EVs.

Free Research Field

分析化学

Academic Significance and Societal Importance of the Research Achievements

容易に採取可能な涙液中の細胞外小胞(ＥＶｓ)の高感度検出により、卵巣がんの早期発見が可能になれば、これまで見過ごされてきた罹患初期の情報を得ることができ、学術的にも高い意義がある。さらに、涙液中のＥＶｓをバイオマーカーとし、卵巣がんの非侵襲分析法が可能となれば、進行したがんの動態の把握のみならず、早期診断あるいは予後診断のためにも有効であると考えられ、社会的にも波及効果がある。