Construction of RNA dynamics science and RNA-targeted drug assay systems

2023 Fiscal Year Final Research Report

• Project/Area Number: 21H02059
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 37010:Bio-related chemistry
• Research Institution: Tokyo Institute of Technology (2022-2023); Osaka University (2021)
• Principal Investigator: KAWAI KIYOHIKO 東京工業大学, 生命理工学院, 教授 (50314422)
• Co-Investigator(Kenkyū-buntansha): 田邉 一仁 青山学院大学, 理工学部, 教授 (40346086)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: RNA / 光化学 / 1分子計測 / 核酸

Outline of Final Research Achievements

In this study, we developed a method for measuring RNA structural transition based on single-molecule fluorescence observations, which allows the dynamics of heterogeneous RNA structures to be investigated with μsec time resolution from very small amounts of RNA, and we used the developed method to deepen the science on the impact of drug binding on RNA dynamics. Specifically, single-molecule measurements of RNA dynamics and structure based on two types of blinking observations were developed. We developed a method for measuring RNA dynamics using blinking by triplet-triplet energy transfer (TTET). A method for detecting point mutations in RNA structure using blinking by electron transfer within nucleic acids was developed.

Free Research Field

光化学

Academic Significance and Societal Importance of the Research Achievements

RNAのダイナミクス、および、RNA配列中の点変異を1分子測定できる手法を開発しました。本手法は、1分子測定に基づくため、貴重な生体資料を用いた測定が可能となります。また、ＰＣＲや検査キットに必要な増幅操作が必要ないため、増幅に要していた時間とコストを削減できます。その場測定が可能であるため、病理切片等におけるその場観察が可能となり、診断や分析に幅広く応用可能な手法として期待されます。