Molecular probe for the evaluation of ppGpp function in eukaryote

2023 Fiscal Year Final Research Report

• Project/Area Number: 21H02075
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 37030:Chemical biology-related
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: SEIO KOHJI 東京工業大学, 生命理工学院, 教授 (20313356)
• Co-Investigator(Kenkyū-buntansha): 増田 真二 東京工業大学, 生命理工学院, 教授 (30373369)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: ppGpp / 緊縮応答 / 分子プローブ / 植物

Outline of Final Research Achievements

In this study, we aimed to develop a technique to isolate and identify ppGpp-binding proteins from eukaryotes by creating affinity beads immobilized with ppGpp and analyzing the function of the identified proteins. The ultimate goal was to establish a methodology to elucidate the mechanism of ppGpp function expression in eukaryotic cells. We successfully created two molecular probes with biotin within their molecules and conducted experiments using one of them to identify ppGpp-binding proteins, resulting in the acquisition of several candidate proteins

Free Research Field

生体関連化学

Academic Significance and Societal Importance of the Research Achievements

本研究では植物の光合成の制御分子であるppGppに関して、その誘導体の合成法の開発とその植物での機能探索のための研究を行った。本研究の成果はバイオテクノロジーに有用なppGppの化学的合成に関する知見を提供するとともに、植物の機能制御に関する新しい視点を提供するものとして学術的・社会的に意義が大きい。