2023 Fiscal Year Final Research Report
Regulatory mechanisms of membrane trafficking and plant growth in response to nutrient availability
Project/Area Number |
21H02150
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Review Section |
Basic Section 38060:Applied molecular and cellular biology-related
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Research Institution | Hokkaido University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
佐藤 長緒 北海道大学, 理学研究院, 准教授 (50609724)
高木 純平 北海道大学, 理学研究院, 助教 (80740331)
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Project Period (FY) |
2021-04-01 – 2024-03-31
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Keywords | 環境応答 / 翻訳後修飾 |
Outline of Final Research Achievements |
In this study, we investigated the molecular mechanism regulating plant responses to C/N-nutrient availability. We carried out the detailed analyses about the post-translational modifications on the membrane trafficking factors. As results, we found the TGN-localized SNARE SYP61 is ubiquitinated in plant, especially by K63-chain, which is responsive to C/N-nutrient availability in Arabidopsis seedlings. In addition, we identified a ARF-GEF protein as a new candidate of C/N-related membrane trafficking factor. We found the loss-of function mutant of the ARF-GEF showed insensitive phenotype to C/N-nutrient stress. I was also suggested that the ARF-GEF protein might promote vesicle trafficking under C/N-nutrient stress conditions.
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Free Research Field |
植物科学
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Academic Significance and Societal Importance of the Research Achievements |
植物のC/N応答は,農作物の光合成活性や収量向上という観点からも重要な生理現象である。本研究から,これまで分かっていなかった細胞内物質輸送制御とC/N応答の関連性について明らかになった。本研究成果は,作物収量を制御する分子基盤の理解に貢献する。
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