2023 Fiscal Year Final Research Report
Creation of novel garden stock resources by combination of mutagenesis, genomics, and transformant
Project/Area Number |
21H02183
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Review Section |
Basic Section 39030:Horticultural science-related
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Research Institution | Shizuoka University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
富永 晃好 静岡大学, 農学部, 助教 (50776490)
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Project Period (FY) |
2021-04-01 – 2024-03-31
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Keywords | ストック / 遺伝子組換え / ゲノム編集 / 突然変異 / 花型 / 花色 |
Outline of Final Research Achievements |
In this study, we revealed genomic sequences of common stock (Matthiola incana). And we established Agrobacterium-mediated transformation system in common stock. The overexpression of MiAG in transgenic plants were exhibited the reduced floral size. On the other hands, the transgenic plants with chimeric repressor of MiSEP3 were exhibited increased floral size, resulted in proliferation flower phenotype as termed “Pop-up”. The irradiation of Ion beam for mature seeds of common stock was induced emergency of several mutants with narrow petals, increased floral axis, or increased petal number. We demonstrated new floral breeding system combined genomics, molecular genetics and mutagenesis.
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Free Research Field |
花卉園芸学
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Academic Significance and Societal Importance of the Research Achievements |
ストックは、冬季に加温を必要としない「省エネ花卉」として注目されている。この栽培上の利点を生かしたうえで、バラやカーネーションのように消費者に魅力ある花形質を有する品種を開発することが求められている。しかし、日本のストック育種は成熟域に達しており、品種開発が停滞している。本研究でストックにおいて、ゲノミックス育種、突然変異育種、形質転換/ゲノム編集育種という新たな育種の扉を開いた。これらの研究成果は、ストックの国内育種の活性化と、需要拡大に繋がることが期待できる。
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