2023 Fiscal Year Final Research Report

Identification of niche cells for supporting colonization, proliferation, and differentiation of xenogenic donor germ cells in recipient gonads.

Research Project

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Project/Area Number	21H02283
Research Category	Grant-in-Aid for Scientific Research (B)
Allocation Type	Single-year Grants
Section	一般
Review Section	Basic Section 40040:Aquatic life science-related
Research Institution	Kanazawa University
Principal Investigator	Takeuchi Yutaka 金沢大学, 生命理工学系, 教授 (70418680)
Co-Investigator(Kenkyū-buntansha)	小林功金沢大学, 生命理工学系, 准教授 (30774757) 山田敏之長崎県総合水産試験場, 種苗量産技術開発センター, 科長 (40443345) 濱崎将臣長崎県総合水産試験場, 種苗量産技術開発センター, 主任研究員 (50506160)
Project Period (FY)	2021-04-01 – 2024-03-31
Keywords	生殖幹細胞 / 移植 / 精巣 / 卵巣
Outline of Final Research Achievements	The surrogate broodstock technology is an interspecies or intraspecies germline stem cell transplantation in which germline stem cells are obtained from a donor fish and transplanted into a surrogate parent fish (recipient) to obtain gametes derived from the donor. In this technology, it is essential to develop a technique to remove germline stem cells from the host. Noting that X-ray irradiation is used in hematopoietic stem cell transplantation research as a technique to temporarily remove hematopoietic stem cells from the recipient to improve transplantation efficiency, we developed a technique to temporarily remove germline stem cells of fish (zebrafish and tiger pufferfish) in a similar manner. In this study, we evaluated the effects of using X-irradiation as a new means of removing endogenous germ cells from adult fish hosts by histological observation of survival and gonads of test fish, gonadal indices, and molecular biological techniques.
Free Research Field	魚類発生工学
Academic Significance and Societal Importance of the Research Achievements	2000年代初頭に東京海洋大学（吉崎悟朗教授）で開発された魚類の生殖幹細胞移植技術は、有用品種や絶滅危惧種の繁殖補助技術として、世界中で広く研究開発されており、一部は水産養殖業において社会実装されている。様々な魚種で成功例が報告され知見が蓄積されているなかで、ドナー種に対してどのような宿主種を選べばよいかについては手探りの状態である。本研究では、魚類の生殖細胞移植においてドナー種に対する免疫拒絶反応の抑制と宿主の内在性生殖幹細胞を除去するためのX線照射技術を開発した。