2023 Fiscal Year Final Research Report
Studies on DNA methylation-based regulation of human cardiomyocyte developmental differentiation mechanisms
Project/Area Number |
21H02381
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Review Section |
Basic Section 42030:Animal life science-related
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Research Institution | University of Miyazaki |
Principal Investigator |
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Project Period (FY) |
2021-04-01 – 2024-03-31
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Keywords | ヒトiPS細胞 / 分化指向性 / 心筋分化 / DNAメチル化 |
Outline of Final Research Achievements |
Human iPSCs are a useful tool for studying cardiomyogenesis, but the differentiation propensity of human iPSCs has become an issue. In this study, we attempted to identify factors that inhibit cardiomyocyte differentiation based on comprehensive DNA methylation data obtained from undifferentiated human iPSCs and differentiation induction experiments. As a result, we identified some DNA methylation variable regions that correlate with the efficiency of induction of differentiation into cardiac cells, and found characteristic changes in chromatin structure. It was suggested that the abnormal epigenetic status of these genomic regions inhibits differentiation into cardiomyocytes. The evaluation of DNA methylation status of the DNA methylation variable regions identified in this study is useful as a new biomarker to predict the cardiomyocyte differentiation potential of undifferentiated human iPSCs.
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Free Research Field |
幹細胞生物学
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Academic Significance and Societal Importance of the Research Achievements |
本研究によって未分化状態のヒトiPS細胞における心筋分化に相関するDNAメチル化可変領域を同定することができた。本研究成果は、心筋発生分化の基礎的理解が進むだけでなく、ヒトiPS細胞の株間差を分子レベルで理解する基盤情報となる。また、未分化時にヒトiPS細胞の心筋分化効率を事前評価できるバイオマーカーとしても有用であり、ヒトiPS細胞の品質評価の新たな指標として再生医療研究へ大きく貢献できる成果である。
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