2023 Fiscal Year Final Research Report
Elucidation of the mechanism of pancreatic beta-cell failure in diabetes using comparative biology-based approach
| Project/Area Number |
21H02390
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 42040:Laboratory animal science-related
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
岡村 匡史 国立研究開発法人国立国際医療研究センター, その他部局等, 実験動物管理室長 (00333790)
須山 幹太 九州大学, 生体防御医学研究所, 教授 (70452365)
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Keywords | 疾患モデル / 糖尿病 / インスリン / 膵β細胞障害 / ゲノム編集 |
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| Outline of Final Research Achievements |
In this study, by using several spontaneous animal models of type 2 diabetes with pancreatic beta-cell failure, we performed genetic analysis and exome analysis to identify diabetes susceptibility genes and verified the relationship of the candidate gene with pathophysiology by CRISPR/Cas9-mediated genome editing system. We also performed transcriptome and proteome analyses of the pancreatic islets of these models to identify pathways involved in the onset of pancreatic beta-cell failure. Together with the information of humans, we clarified a part of mechanisms involved in the development and progression of pancreatic beta-cell failure.
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| Free Research Field |
分子遺伝学、分子糖尿病学、実験動物学
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| Academic Significance and Societal Importance of the Research Achievements |
複数のマウスおよびラットの自然発症糖尿病モデルを駆使した本研究により明らかとなった膵β細胞障害の病態発症・進展機構は、ヒト糖尿病における膵β細胞障害の病態発症・進展機構の解明や新規のメカニズムに基づく治療薬の開発の基盤となると考えられる。本研究は、同様の研究手法が適用可能な様々の研究分野において疾患モデルの有用性を実証するものである。
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