2023 Fiscal Year Final Research Report
Study of tissue development based on analysis of Wnt dynamics
| Project/Area Number |
21H02498
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 44020:Developmental biology-related
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| Research Institution | National Institutes of Natural Sciences |
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Principal Investigator |
Takada Shinji 大学共同利用機関法人自然科学研究機構(機構直轄研究施設), 生命創成探究センター, 教授 (60206753)
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Keywords | 遺伝子 / 細胞 / 発生・分化 / シグナル伝達 |
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| Outline of Final Research Achievements |
Secretory signal proteins, including Wnt, are considered to act through secretion and diffusion from producing cells, but there is insufficient data to support this theory. Therefore, in this study, we aimed to understand the molecular basis that regulates Wnt diffusivity and to experimentally clarify the significance of diffusion. As a result, the specificity of Wnt complex formation and the influence of interactions with cell surface molecules on Wnt dynamics were clarified, and analysis using mouse embryos in which Wnt diffusion does not occur revealed the significance of Wnt diffusion. Based on the above multifaceted analysis, a new model of spatial control during tissue development was proposed.
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| Free Research Field |
発生生物学、分子生物学
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| Academic Significance and Societal Importance of the Research Achievements |
Wntの組織内伝達機構に関する研究は、ショウジョウバエを主なモデルにして精力的な解析が行われてきた。その結果、Wgが細胞外小胞により運搬されるというモデルが提唱されている。一方、これらWnt拡散型のモデルと対峙する形で、非拡散性のWntであっても組織の領域形成を起こしうるという報告がなされ、Wntの拡散の意義とその制御機構は現在大きな議論の対象になっていた。本研究はWntの拡散の重要性を生化学的解析を含む多面的な解析により検討したものであり、その結果はWntシグナルの組織内伝達機構の理解に大きく寄与する。
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