Elucidation of last common ancestor of land plants based on meristem related genes

2023 Fiscal Year Final Research Report

• Project/Area Number: 21H02549
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 45030:Biodiversity and systematics-related
• Research Institution: Kanazawa University
• Principal Investigator: Nishiyama Tomoaki 金沢大学, 疾患モデル総合研究センター, 助教 (50390688)
• Co-Investigator(Kenkyū-buntansha): 関本 弘之 日本女子大学, 理学部, 教授 (20281652) ; 坂山 英俊 神戸大学, 理学研究科, 准教授 (60391108) ; 榊原 恵子 立教大学, 理学部, 准教授 (90590000)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: ナガサキツノゴケ / シャジクモ / ヒメミカヅキモ / YABBY / LEAFY

Outline of Final Research Achievements

Closterium yabby knockout candidate strains were obtained. Anthoceros agrestis KNOX2 and BELL ectopic expression strains were obtained and their phenotypes were observed. Chara braunii LEAFY cDNA containing complete coding sequence was cloned and its nucleotide sequence was determined. The cDNA were transformed to A. agrestis for ectopic expression. CRISPR-Cas9 construct with A. agrestis U6 promoter candidate was introduced to A. agrestis, but editing was not confirmed. Confirmation of expression of each component should be performed. A new promoter terminator combination for C. braunii was confirmed to be able to drive an exogeneous reporter gene by particle bombardment.

Free Research Field

植物進化学

Academic Significance and Societal Importance of the Research Achievements

コケ植物、シャジクモ藻類のゲノム解読からわかってきた被子植物のメリステム制御に関わる遺伝子の機能解明を進めている。この研究により、陸上植物の共通祖先はどのようなものであったのか、藻類からどのようにして陸上植物が成立し、陸上植物が多様化したかの過程についてより具体的に推定するのにつながる。またこれらの生物での遺伝子機能解析技術の向上は他の遺伝子の機能解析にも寄与する。