2023 Fiscal Year Final Research Report
Novel strategies for the treatment of solid tumors with CAR and CIML NK cells
| Project/Area Number |
21H02789
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 50020:Tumor diagnostics and therapeutics-related
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
久保 智洋 札幌医科大学, 医学部, 助教 (00634669)
大須賀 崇裕 札幌医科大学, 医学部, 助教 (40619714)
田中 信悟 札幌医科大学, 医学部, 助教 (60561024)
加藤 淳二 札幌医科大学, 医学部, 教授 (20244345)
濱口 孝太 札幌医科大学, 医学部, 研究員 (50866613)
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Keywords | CAR-NK |
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| Outline of Final Research Achievements |
Using piggyBac transposon plasmid vector, CAR-CIML NK cells were generated and proved to have potent cytotoxic activity upon recognition of target cells in vitro. Vector transduction by electroporation was highly cytotoxic, and no proliferation of CAR-CIML NK cells was observed in vitro. In the future, it is necessary to develop a method to reduce the cytotoxicity of vector transduction and to examine the anti-tumor effect of CAR-CIML NK cells in an in vivo mouse model.
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| Free Research Field |
腫瘍免疫
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| Academic Significance and Societal Importance of the Research Achievements |
本研究ではCIML NK細胞の概念をCAR細胞に応用し、膵癌細胞株に対して高い抗腫瘍効果を発揮することを証明し、今後の新規固形癌治療に繋がる可能性がある。
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