Mechanism underlying initiation of bone marrow hematopoiesis in neonate

2023 Fiscal Year Final Research Report

• Project/Area Number: 21H02953
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 54010:Hematology and medical oncology-related
• Research Institution: Kumamoto University
• Principal Investigator: Takizawa Hitoshi 熊本大学, 国際先端医学研究機構, 特別招聘教授 (10630866)
• Co-Investigator(Kenkyū-buntansha): 黒滝 大翼 熊本大学, 国際先端医学研究機構, 特任准教授 (10568455) ; 波江野 洋 東京理科大学, 研究推進機構生命医科学研究所, 准教授 (70706754)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: 造血幹細胞

Outline of Final Research Achievements

The hematopoietic stem cells (HSCs) that maintain the hematopoietic system cross various hematopoietic tissues in the early stages of development, and ultimately settle in the bone marrow (BM), the site of adult hematopoiesis. Although BM hematopoiesis is thought to begin before birth, the detailed mechanism by which HSCs adhere to the BM and initiate adult hematopoiesis remains unclear. Our previous single-cell analysis revealed the presence of three HSC subsets with different self-renewal and differentiation abilities in the perinatal BM. In this study, we aim to elucidate the dynamic mechanism of BM hematopoiesis initiation that occurs during the perinatal period, focusing on the origin and functional relationships of different HSCs, control by undergoing cell maturation, and contribution to adult hematopoiesis. The insights gained will be useful in regenerative medicine such as peripheral blood mobilization of HSCs and BM transplantation.

Free Research Field

血液学

Academic Significance and Societal Importance of the Research Achievements

組織を維持する幹細胞の機能特性や成体組織に向かう器官形成の一端を明らかにすることは、骨髄以外の幹細胞組織にも新たな生物学的視点を持ち込み学術的価値が高い。固有の実験材料、最新シングルセル解析技術、これまで構築してきた国内外の共同研究者との研究連携を活用することで、十分な国際研究競争力をもった大きな研究成果を残せることが期待される。本研究で得られる知見は造血幹細胞の髄外動員（Wright DE, Science 2001）や効率的な骨髄生着など再生医療に有用な知見をもたらすだろう