脳と免疫系相互作用の観点からの多階層的研究による精神疾患病態解明

2022 Fiscal Year Annual Research Report

• Project/Area Number: 21H04815
• Research Institution: Nagoya University
• Principal Investigator: 尾崎 紀夫 名古屋大学, 医学系研究科, 特任教授 (40281480)
• Co-Investigator(Kenkyū-buntansha): 和氣 弘明 名古屋大学, 医学系研究科, 教授 (90455220) ; 大野 欽司 名古屋大学, 医学系研究科, 教授 (80397455) ; 野田 幸裕 名城大学, 薬学部, 教授 (90397464) ; 財津 桂 近畿大学, 生物理工学部, 教授 (30700546)
• Project Period (FY): 2021-04-05 – 2024-03-31
• Keywords: 22q11.2欠失 / 全ゲノム解析 / 患者死後脳 / ミクログリア / 腸内細菌叢 / 短鎖脂肪酸 / 周産期免疫活性化 / 炎症性メディエーター

Outline of Annual Research Achievements

代表尾崎は、ゲノム因子と免疫系の相互作用による脳病態を解明するため、22q11.2欠失を代表的なモデルとし、①当該バリアント等の精神疾患患者を対象とした全ゲノム解析・臨床表現型解析、②当該バリアント患者由来iPS細胞由来ミクログリアの機能解析、③当該バリアントモデルマウスを対象とした全脳c-fos解析、④当該バリアント患者死後脳収集を引き続き実施した。
分担和氣は、統合失調症の周産期免疫活性化モデル（胎児期TLR7注入モデル）を確立し、ストレスを付加することによる行動異常の観察に成功、現在神経回路解析を進めている。一方で感覚統合のメカニズム解明のため、ミクログリアの異種感覚の統合に対する寄与を明らかにし、論文化した。
分担大野は、22q11.2欠失を有する患者、睡眠障害や摂食障害の患者便からDNA抽出後、MiSeqを用いて16S rRNA V3-V4のamplicon-seqを行い、QIIME2パイプラインによる細菌叢解析を実施。さらに便中短鎖脂肪酸分析により摂食障害の患者便ではイソ酪酸およびイソ吉草酸が上昇しており、腸内細菌叢の変化が示唆された。
分担野田は、ポリイノシン：ポリシチジル酸（PolyI:C）を投与した仔マウスにおいて増加していたプロスタグランジンE2（PGE2）の高次脳機能障害への影響を検討した。その結果、PolyI:Cによる新生仔期免疫活性化をおこなったマウスにおいて、TNF-αやIL-6、MCP-1、COX-2の遺伝子発現が増加。PGE2による新生仔期の免疫を活性化させたマウスでは、成体期において社会性、認知機能および感覚情報処理などの高次脳機能の障害が認められた。
分担財津は、PiTMaPメソッドの見直しを行い、キヌレニン経路代謝物の分析条件を検討した。LPSで炎症惹起したマウスの脳試料等を採取し、腹腔内液中IL-1βの上昇が確認された。

Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

ゲノム解析・iPS細胞解析・モデルマウス解析・死後脳解析のいずれのパートも新たな検体及びデータが得られ、論文化もできていることから概ね順調に進んでいると考えている。また異種感覚統合についても成果を論文化することができ、さらに研究を進めている。22q11.2欠失モデルマウスの解析については、繁殖に遅れが生じていたため、野生型マウスの脳内免疫活性化したモデルマウス（新生仔期免疫活性化マウス）の分子病態の検討に切り替えた。現在、22q11.2欠失モデルマウスの繁殖が安定し、当該マウスに対する新生仔期免疫活性化を順次実施し行動解析を進めている。次年度中旬までに全ての解析を終えることを目標に計画することでこれまでの遅れを取り戻していく予定である。

Strategy for Future Research Activity

代表尾崎は、精神疾患患者のゲノム・血液・死後脳・臨床表現型については引き続き収集し、適宜解析を進める。22q11.2欠失等の発症に関連するゲノムバリアントを有する患者からはiPS細胞を樹立するとともに、表現型解析についても進めていく。モデルマウスについても空間トランスクリプトーム解析等新しい研究手法を取り入れながら機能解析を進めていく。
分担和氣は、今後異種感覚の統合領域における免疫学的なミクログリアのシナプスに対する寄与を明らかにする。さらに免疫学的な観点から統合失調症の免疫モデル（胎児期TLR7注入モデル）の感覚統合に対するメカニズムの変化を明らかにしていく。
分担大野は、22q11.2欠失を有する患者、睡眠障害、摂食障害の患者において変化をする腸内細菌叢と短鎖脂肪酸の変化の臨床的な意義づけを今後探る。
分担野田は、22q11.2欠失モデルマウスの新生仔期に脳内免疫系を活性化させ、各種行動試験を用いて情動・社会性・認知機能・感覚情報処理機能を計画にしたがって検討する予定である。

Research Products

• [Journal Article] Microglia enable cross-modal plasticity by removing inhibitory synapses (2023)
  • Author(s): Hashimoto Akari、Kawamura Nanami、Tarusawa Etsuko、Takeda Ikuko、Aoyama Yuki、Ohno Nobuhiko、Inoue Mio、Kagamiuchi Mai、Kato Daisuke、Matsumoto Mami、Hasegawa Yoshihiro、Nabekura Junichi、Schaefer Anne、Moorhouse Andrew J.、Yagi Takeshi、Wake Hiroaki
  • Journal Title: Cell Reports Volume: 42 Pages: 112383～112383
  • DOI: 10.1016/j.celrep.2023.112383
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Shedding light on latent pathogenesis and pathophysiology of mental disorders: the potential of iPS cell technology (2023)
  • Author(s): Arioka, Y. Okumura, H. Sakaguchi, H. Ozaki, N.
  • Journal Title: Psychiatry Clin Neurosci Volume: - Pages: -
  • DOI: 10.1111/pcn.13545
  • Peer Reviewed / Open Access
• [Journal Article] The genetic architecture of schizophrenia: review of large-scale genetic studies (2023)
  • Author(s): Kato, H. Kimura, H. Kushima, I. Takahashi, N. Aleksic, B. Ozaki, N.
  • Journal Title: J Hum Genet Volume: 68 Pages: 175-182
  • DOI: 10.1038/s10038-022-01059-4
  • Peer Reviewed / Open Access
• [Journal Article] Clinical manifestations of schizophrenia in four patients with variants in voltage-gated calcium channel-encoding genes: a case series (2023)
  • Author(s): Lo, T. Kushima, I. Aleksic, B. Yoshimi, A. Someya, T. Watanabe, Y. Ozaki, N.
  • Journal Title: Psychiatry Clin Neurosci Volume: 77 Pages: 57-59
  • DOI: 10.1111/pcn.13494
  • Peer Reviewed / Open Access
• [Journal Article] Astrotactin2 (ASTN2) regulates emotional and cognitive functions by affecting neuronal morphogenesis and monoaminergic systems (2023)
  • Author(s): Ito, T. Yoshida, M. Aida, T. Kushima, I. Hiramatsu, Y. Ono, M. Yoshimi, A. Tanaka, K. Ozaki, N. Noda, Y.
  • Journal Title: J Neurochem Volume: 165 Pages: 211-229
  • DOI: 10.1111/jnc.15790
  • Peer Reviewed
• [Journal Article] Myelinated axon as a plastic cable regulating brain functions (2023)
  • Author(s): Sugio S,Kato D, Wake H.
  • Journal Title: Neuroscience Research Volume: 187 Pages: 45-51
  • DOI: 10.1016/j.neures.2022.11.002
  • Peer Reviewed
• [Journal Article] Impaired gating of γ- and ε-AChR respectively causes Escobar syndrome and fast channel myasthenia (2023)
  • Author(s): Shen XM, Nakata T, Mizuno S, Imoto I, Selcen D, Ohno K, Engel AG.
  • Journal Title: Ann Clin Transl Neurol Volume: - Pages: -
  • DOI: 10.1002/acn3.51756
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Evaluation of Human-Induced Pluripotent Stem Cells Derived from a Patient with Schwartz Jampel Syndrome Revealed Distinct Hyperexcitability in the Skeletal Muscles (2023)
  • Author(s): Yamashita Y, Nakada S, Nakamura K, Sakurai H, Ohno K, Goto T, Mabuchi Y, Akazawa C, Hattori N, Arikawa-Hirasawa E.
  • Journal Title: Biomedicines Volume: 11 Pages: 814
  • DOI: 10.3390/biomedicines11030814
  • Peer Reviewed / Open Access
• [Journal Article] Machine learning models predict delayed hyponatremia post-transsphenoidal surgery using clinically available features (2023)
  • Author(s): Fuse Y, Nishiwaki H, Imaizumi T, Nagata Y, Ohno K, Saito R.
  • Journal Title: Pituitary Volume: - Pages: -
  • DOI: 10.1007/s11102-023-01311-w
  • Peer Reviewed
• [Journal Article] Clinical and pathologic features of congenital myasthenic syndromes caused by 35 genes a comprehensive review (2023)
  • Author(s): Ohno K, Ohkawara B, Shen X-M, Selcen D, Engel AG.
  • Journal Title: Int J Mol Sci Volume: 24 Pages: 3730
  • DOI: 10.3390/ijms24043730
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The On/off History of Hydrogen in Medicine: Will the interest persist this time around? (2023)
  • Author(s): LeBaron TW, Ohno K, Hancock JT.
  • Journal Title: Oxygen Volume: 3 Pages: 143-162
  • DOI: 10.3390/oxygen3010011
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Gut Microbiota Changes and Parkinson’s Disease: What Do We Know, Which Avenues Ahead (2023)
  • Author(s): Hirayama M, Ohno K.
  • Journal Title: Gut Microbiota in Aging and Chronic Diseases Volume: - Pages: 257-278
  • DOI: 10.1007/978-3-031-14023-5_13
  • Peer Reviewed
• [Journal Article] Bioinformatics and data science for mass spectrometry data analysis (2023)
  • Author(s): Zaitsu K, Eguchi S, Iguchi A.
  • Journal Title: Medical Mass Spectrometry Volume: - Pages: -
  • Peer Reviewed
• [Journal Article] X chromosome aneuploidies and schizophrenia: association analysis and phenotypic characterization (2022)
  • Author(s): Kushima, I. Aleksic, B. Kimura, H. Nakatochi, M. Lo, T. Ikeda, M. Arai, M. Hashimoto, R. Numata, S. Okamura, Y. Obara, T. Inada, T. Ozaki, N.
  • Journal Title: Psychiatry Clin Neurosci Volume: 76 Pages: 667-673
  • DOI: 10.1111/pcn.13474
  • Peer Reviewed / Open Access
• [Journal Article] Turner syndrome presenting with idiopathic regression: A case report (2022)
  • Author(s): Mizobuchi, K. Kushima, I. Kato, H. Miyajima, M. Kimura, H. Ozaki, N.
  • Journal Title: Psychiatry Clin Neurosci Volume: 76 Pages: 680-682
  • DOI: 10.1111/pcn.13483
  • Peer Reviewed / Open Access
• [Journal Article] Sequencing of selected chromatin remodelling genes reveals increased burden of rare missense variants in ASD patients from the Japanese population (2022)
  • Author(s): Lo, T. Kushima, I. Aleksic, B. Kato, H. Nawa, Y. Hayashi, Y. Otgonbayar, G. Kimura, H. Arioka, Y. Mori, D. Ozaki, N.
  • Journal Title: Int Rev Psychiatry Volume: 34 Pages: 154-167
  • DOI: 10.1080/09540261.2022.2072193
  • Peer Reviewed
• [Journal Article] Psychiatric patients with a de novo 17q12 deletion: Two case reports (2022)
  • Author(s): Kushima, I. Uematsu, M. Ishizuka, K. Aleksic, B. Ozaki, N.
  • Journal Title: Psychiatry Clin Neurosci Volume: 76 Pages: 345-347
  • DOI: 10.1111/pcn.13367
  • Peer Reviewed / Open Access
• [Journal Article] Memantine ameliorates the impairment of social behaviors induced by a single social defeat stress as juveniles (2022)
  • Author(s): Yoshida, M. Hasegawa, S. Taniguchi, M. Mouri, A. Suzuki, C. Yoshimi, A. Mamiya, T. Ozaki, N. Noda, Y.
  • Journal Title: Neuropharmacology Volume: 217 Pages: 109208
  • DOI: 10.1016/j.neuropharm.2022.109208
  • Peer Reviewed
• [Journal Article] Involvement of cerebellar and subcortical connector hubs in schizophrenia (2022)
  • Author(s): Yamamoto, M. Bagarinao, E. Shimamoto, M. Iidaka, T. Ozaki, N.
  • Journal Title: Neuroimage Clin Volume: 35 Pages: 103140
  • DOI: 10.1016/j.nicl.2022.103140
  • Peer Reviewed / Open Access
• [Journal Article] Exome sequencing of Japanese schizophrenia multiplex families supports the involvement of calcium ion channels (2022)
  • Author(s): Toyama, M. Takasaki, Y. Branko, A. Kimura, H. Kato, H. Nawa, Y. Kushima, I. Ishizuka, K. Shimamura, T. Ogi, T. Ozaki, N.
  • Journal Title: PLoS One Volume: 17 Pages: e0268321
  • DOI: 10.1371/journal.pone.0268321
  • Peer Reviewed / Open Access
• [Journal Article] Exome sequencing analysis of Japanese autism spectrum disorder case-control sample supports an increased burden of synaptic function-related genes (2022)
  • Author(s): Kimura, H. Nakatochi, M. Aleksic, B. Guevara, J. Toyama, M. Hayashi, Y. Kato, H. Kushima, I. Morikawa, M. Ishizuka, K. Okada, T. Tsurusaki, Y. Fujita, A. Miyake, N. Ogi, T. Takata, A. Matsumoto, N. Buxbaum, J. Ozaki, N. Sebat, J.
  • Journal Title: Transl Psychiatry Volume: 12 Pages: 265
  • DOI: 10.1038/s41398-022-02033-6
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Cross-Disorder Analysis of Genic and Regulatory Copy Number Variations in Bipolar Disorder, Schizophrenia, and Autism Spectrum Disorder (2022)
  • Author(s): Kushima, I. Nakatochi, M. Aleksic, B. Okada, T. Kimura, H. Kato, H. Morikawa, M. Inada, T. Ishizuka, K. Torii, Y. Nakamura, Y. Tanaka, S. Imaeda, M. Takahashi, N. Yamamoto, M. Iwamoto, K. Nawa, Y. Ogawa, N. Iritani, S. Hayashi, Y. Lo, T. Otgonbayar, G. Furuta, S. Iwata, N. Ikeda, T. Arioka, Y. Mori, D. Ozaki, N, et al.
  • Journal Title: Biol Psychiatry Volume: 92 Pages: 362-374
  • DOI: 10.1016/j.biopsych.2022.04.003
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Contribution of copy number variations to the risk of severe eating disorders (2022)
  • Author(s): Kushima, I. Imaeda, M. Tanaka, S. Kato, H. Oya-Ito, T. Nakatochi, M. Aleksic, B. Ozaki, N.
  • Journal Title: Psychiatry Clin Neurosci Volume: 76 Pages: 423-428
  • DOI: 10.1111/pcn.13430
  • Peer Reviewed / Open Access
• [Journal Article] Elucidation of the neurological effects of clothianidin exposure at the no-observed-adverse-effect level (NOAEL) using two-photon microscopy in vivo imaging (2022)
  • Author(s): Nishi M, Sugio S, Hirano T, Kato D, Wake H, Shoda A, Murata M, Ikenaka Y, Tabuchi Y, Mantani Y, Yokoyama T, Hoshi N.
  • Journal Title: Journal of Veterinary Medical Science Volume: 84 Pages: 585-592
  • DOI: 10.1292/jvms.22-0013
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Lighting up cosmic neuronal networks with transformative in vivo calcium imaging (2022)
  • Author(s): Murayama M, Wake H.
  • Journal Title: Neuroscience Research Volume: 179 Pages: 1-2
  • DOI: 10.1016/j.neures.2022.04.007
  • Peer Reviewed
• [Journal Article] A Piezo1/KLF15/IL-6 axis mediates immobilization-induced muscle atrophy (2022)
  • Author(s): Hirata Y, Nomura K, Kato D, Tachibana Y, Niikura T, Uchiyama K, Hosooka T, Fukui T, Oe K, Kuroda R, Hara Y, Adachi T, Shibasaki K, Wake H, Ogawa W.
  • Journal Title: Journal of Clinical Investigation Volume: 132 Pages: 1-13
  • DOI: 10.1172/JCI154611
  • Peer Reviewed
• [Journal Article] Holographic microscope and its biological application (2022)
  • Author(s): Quan X, Kato D, Daria V, Matoba O, Wake H.
  • Journal Title: Neurosci Res Volume: 179 Pages: 57-64
  • DOI: 10.1016/j.neures.2021.10.012
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Controlled activation of cortical astrocytes modulates neuropathic pain-like behaviour (2022)
  • Author(s): Takeda I, Yoshihara K, Cheung DL, Kobayashi T, Agetsuma M, Tsuda M, Eto K, Koizumi S, Wake H, Moorhouse AJ, Nabekura J.
  • Journal Title: Nature Communications Volume: 13 Pages: 4100
  • DOI: 10.1038/s41467-022-31773-8
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Evaluation and Manipulation of Neural Activity using Two-Photon Holographic Microscopy (2022)
  • Author(s): Kato D, Quan X, Tanisumi Y, Guo Z, Morita M, Takiguchi T, Matoba O, Wake H.
  • Journal Title: J Vis Exp Volume: 187 Pages: 64205
  • DOI: 10.3791/64205
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Possible repositioning of an oral anti-osteoporotic drug, Ipriflavone, for treatment of inflammatory arthritis via inhibitory activity of KIAA1199, a novel potent hyaluronidase (2022)
  • Author(s): Koike H, Nishida Y, Shinomura T, Ohkawara B, Ohno K, Zhuo Lisheng, Kimata K, Ushida T, Imagama S
  • Journal Title: Int J Mol Sci Volume: 23 Pages: 4089
  • DOI: 10.3390/ijms23084089
  • Peer Reviewed / Open Access
• [Journal Article] Short chain fatty acids-producing and mucin-degrading intestinal bacteria predict the progression of early Parkinson’s disease (2022)
  • Author(s): Nishiwaki H, Ito M, Hamaguchi T, Maeda T, Kashihara K, Tsuboi Y, Ueyama J, Yoshida T, Hanada H, Takeuchi I, Katsuno M, Hirayama M, Ohno K.
  • Journal Title: npj Parkinsons Dis Volume: 8 Pages: 65
  • DOI: 10.1038/s41531-022-00328-5
  • Peer Reviewed / Open Access
• [Journal Article] Extremely low-frequency pulses of faint magnetic field induce mitophagy to rejuvenate mitochondria (2022)
  • Author(s): Toda T, Ito M Takeda J, Masuda A, Mino H, Hattori N, Mohri K, Ohno K.
  • Journal Title: Commun Biol Volume: 5 Pages: 453
  • DOI: 10.1038/s42003-022-03389-7
  • Peer Reviewed / Open Access
• [Journal Article] Efficacy of auranofin as an inhibitor of desmoid progression (2022)
  • Author(s): Ito K, Nishida Y, Hamada S, Shimizu K, Sakai T, Ohkawara B, Alman BA, Enomoto A, Ikuta K, Koike H, Zhang J, Ohno K, Imagama S.
  • Journal Title: Sci Rep Volume: 12 Pages: 11918
  • DOI: 10.1038/s41598-022-15756-9
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Examination of abnormal alpha-synuclein aggregates in the enteric neural plexus in patients with ulcerative colitis (2022)
  • Author(s): Gibo N, Hamaguchi T, Miki Y, Yamamura T, Nakaguro M, Ito M, Nakamuara M, Kawashima H, Hirayama M, Hirooka Y, Wakabayashi K, Ohno K.
  • Journal Title: J Gastrointestin Liver Dis Volume: 31 Pages: 290-300
  • DOI: 10.15403/jgld-4313
  • Peer Reviewed
• [Journal Article] Gut microbiota in dementia with Lewy bodies (2022)
  • Author(s): Nishiwaki H, Ueyama J, Kashihara K, Ito M, Hamaguchi T, Maeda T, Tsuboi Y, Katsuno M, Hirayama M, Ohno K.
  • Journal Title: npj Parkinsons Dis Volume: 8 Pages: 169
  • DOI: 10.1038/s41531-022-00428-2
  • Peer Reviewed / Open Access
• [Journal Article] Effects of pesticide intake on gut microbiota and metabolites in healthy adults (2022)
  • Author(s): Ueyama J, Hayashi M, Hirayama M, Nishiwaki H, Ito M, Saito I, Tsuboi Y, Isobe T, Ohno K.
  • Journal Title: Int. J Environ Res Public Health Volume: 20 Pages: 213
  • DOI: 10.3390/ijerph20010213
  • Peer Reviewed / Open Access
• [Journal Article] Efficacy of soluble lansoprazole-impregnated beta-tricalcium phosphate for bone regeneration (2022)
  • Author(s): Mishima K, Okabe YT, Mizuno M, Ohno K, Kitoh H, Imagama S.
  • Journal Title: Sci Rep Volume: 12 Pages: 20550
  • DOI: 10.1038/s41598-022-25184-4
  • Peer Reviewed / Open Access
• [Journal Article] Meclozine ameliorates bone mineralization and growth plate structure in a mouse model of X linked hypophosphatemia (2022)
  • Author(s): Kamiya Y, Matsushita M, Mishima K, Ohkawara B, Michigami T, Imagama S, Ohno K, Kitoh H.
  • Journal Title: Exp Ther Med Volume: 25 Pages: 39
  • DOI: 10.3892/etm.2022.11738
  • Peer Reviewed
• [Journal Article] A mutation in DOK7 in congenital myasthenic syndrome forms aggresome in cultured cells, and reduces DOK7 expression and MuSK phosphorylation in patient-derived iPS cells (2022)
  • Author(s): Zhang S, Ohkawara B, Ito M, Huang Z, Zhao F, Nakata T, Takeuchi T, Sakurai H, Komaki H, Kamon M, Araki T, and Ohno K.
  • Journal Title: Hum Mol Genet Volume: 32 Pages: 1511-1523
  • DOI: 10.1093/hmg/ddac306
  • Peer Reviewed / Open Access
• [Journal Article] Reply to the Letter to the Editor “The microbiota in Parkinson's disease: ranking the risk of heart disease” (2022)
  • Author(s): Hirayama M, Ohno K.
  • Journal Title: Ann Nutr Metab Volume: 78 Pages: 119-120
  • DOI: 10.1159/000521993
  • Peer Reviewed / Open Access
• [Journal Article] Electrolyzed Reduced Water: Review I. Molecular hydrogen is the Exclusive Agent Responsible for the Therapeutic Effects (2022)
  • Author(s): LeBaron TW, Sharpe R, Ohno K.
  • Journal Title: Int J Mol Sci Volume: 23 Pages: 14750
  • DOI: 10.3390/ijms232314750
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Electrolyzed Reduced Water: Review II. Safety Concerns and Effectiveness as a Source of Hydrogen Water (2022)
  • Author(s): LeBaron TW, Sharpe R, Ohno K.
  • Journal Title: Int J Mol Sci Volume: 23 Pages: 14508
  • DOI: 10.3390/ijms232314508
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Metabolomics and Data-Driven Bioinformatics Revealed Key Maternal Metabolites Related to Fetal Lethality via Di(2-ethylhexyl)phthalate Exposure in Pregnant Mice (2022)
  • Author(s): Zaitsu K, Asano T, Kawakami D, Chang J, Hisatsune K, Taniguchi M, Iguchi A.
  • Journal Title: ACS Omega Volume: 7 Pages: 23717-23726
  • DOI: 10.1021/acsomega.2c02338
  • Peer Reviewed
• [Journal Article] Preliminary study to classify mechanisms of mitochondrial toxicity by in vitro metabolomics and bioinformatics (2022)
  • Author(s): Hibino Y, Iguchi A, Zaitsu K.
  • Journal Title: Toxicol Appl Pharmacol Volume: - Pages: 116316
  • DOI: 10.1016/j.taap.2022.116316
  • Peer Reviewed
• [Presentation] 多細胞回路動態の計測と操作 (2023)
  • Author(s): 和氣弘明
  • Organizer: 量子制御生命研究会
• [Presentation] 光で階層的に神経・グリア回路活動を叙述し、制御する研究を目指して (2023)
  • Author(s): 和氣弘明
  • Organizer: レーザー学会年次大会
• [Presentation] ホログラム刺激による神経回路再編の人為的創出 (2023)
  • Author(s): 和氣弘明
  • Organizer: CRESTオプトバイオ第7回領域会議
• [Presentation] 多細胞回路動態の計測と操作を目指して (2023)
  • Author(s): 和氣弘明
  • Organizer: The Future of Diabetes Conference
  • Invited
• [Presentation] オリゴデンドロサイトのよる神経回路の同期性制御 (2023)
  • Author(s): 和氣弘明
  • Organizer: 第100回日本生理学会大会
• [Presentation] ミクログリアによる脳機能制御と病態の表出 (2023)
  • Author(s): 和氣弘明
  • Organizer: 第128回日本解剖学会総会
• [Presentation] Toward understanding multicellular circuit dynamics (2023)
  • Author(s): 和氣弘明
  • Organizer: Korea Univ Med,Yonsei Univ Med,Yonesei Univ Dent,NIPS 合同シンポジウム
  • Int'l Joint Research
• [Presentation] Meclozine ameliorates Fgf2-induced craniofacial bone hyperossification of larval zebrafish by attenuating MAPK pathway (2023)
  • Author(s): Takemoto G, Matsushita M, Ohno K, Imagama S.
  • Organizer: Orthopaedic Research Society 2023 Annual Meeting
  • Int'l Joint Research
• [Presentation] オミクス計測におけるアンビエントイオン化質量分析の応用と展望 (2023)
  • Author(s): 財津桂
  • Organizer: 電子情報通信学会 MEとバイオサイバネティクス研究会
• [Presentation] COVID-19拡大とうつ病・双極性障害：生物心理社会的観点から (2022)
  • Author(s): 尾崎紀夫
  • Organizer: 2022日本うつ病リワーク協会年次大会特別講演
• [Presentation] アルコール使用性障害を高頻度に合併する統合失調症の発症に関わるゲノム変異から病態解明へ：22q11.2欠失を例として (2022)
  • Author(s): 尾崎紀夫
  • Organizer: 2022アルコール・薬物依存合同総会 「アルコール・薬物濫用と関連疾患の医学的解決」
• [Presentation] 精神疾患の連続性を踏まえた個別化医療-Diseasomeの観点から (2022)
  • Author(s): 尾崎紀夫
  • Organizer: 第41回日本精神科診断学会ランチョンセミナー
• [Presentation] 精神疾患及び向精神薬の運転技能への影響を考える (2022)
  • Author(s): 尾崎紀夫
  • Organizer: BCNPNPPP4学会合同年会教育講演
• [Presentation] 精神疾患リスクゲノム変異を起点とした多様な試料を用いた病態解明 (2022)
  • Author(s): 尾崎紀夫
  • Organizer: BPCNPNPPP4学会合同年会シンポジウム 精神疾患研究におけるヒトiPSC研究最前線
• [Presentation] 生体イメージングで解き明かすグリア細胞の新規生理機能とその病態への寄与 (2022)
  • Author(s): 和氣弘明
  • Organizer: 第63回日本神経学会学術大会
• [Presentation] 多細胞回路動態の計測と操作 (2022)
  • Author(s): 和氣弘明
  • Organizer: Cardiovascular Research Conference
  • Invited
• [Presentation] 多細胞回路動態の計測と操作 (2022)
  • Author(s): 和氣弘明
  • Organizer: Parkinson's Disease Expert Meeting～新たな治療選択肢を考える～
  • Invited
• [Presentation] ミクログリアの新規生理機能 (2022)
  • Author(s): 和氣弘明
  • Organizer: 第4回量子生命科学会
• [Presentation] Microglia and sensory modality (2022)
  • Author(s): 和氣弘明
  • Organizer: NEURO2022
• [Presentation] 光による神経・グリア生理活動の叙述と制御 (2022)
  • Author(s): 和氣弘明
  • Organizer: 第43回日本炎症・再生医学会
• [Presentation] 光で階層的に神経・グリア回路活動を叙述し、制御する研究を目指して (2022)
  • Author(s): 和氣弘明
  • Organizer: 北海道大学神経内科オンライン講演
  • Invited
• [Presentation] グリア細胞の生理機能と病態への関与 (2022)
  • Author(s): 和氣弘明
  • Organizer: 第16回日本パーキンソン病・運動障害疾患コングレス
  • Invited
• [Presentation] Microglia and sensory modality (2022)
  • Author(s): 和氣弘明
  • Organizer: Glia and Neuroimmune symposium held at Anhui Medical University
  • Invited
• [Presentation] Microglia and sensory modality (2022)
  • Author(s): 和氣弘明
  • Organizer: ISN-APSN2022Biennial Meeting
  • Int'l Joint Research
• [Presentation] Toward understanding multicellular circuit dynamics (2022)
  • Author(s): 和氣弘明
  • Organizer: Heterogeneity of Glia Functions in Development and Disease
  • Int'l Joint Research / Invited
• [Presentation] Physiological and pathological functions of microglia (2022)
  • Author(s): 和氣弘明
  • Organizer: 第95回日本生化学会大会
• [Presentation] Toward understanding multicellular circuit dynamics (2022)
  • Author(s): 和氣弘明
  • Organizer: The 1st Fujita International Symposium on Brain Science
• [Presentation] Microglial removal of inhibitory synapses unleash the multi-sensory potential in the association cortex (2022)
  • Author(s): Wake H, Hashimoto A, Kawamura N, Tarusawa E, Takeda I, Ohno N, Inoue M, Kato D, Aoyama Y, Matsumoto M, Hasegawa Y.
  • Organizer: The Australian Physiological Society annual meeting
  • Int'l Joint Research / Invited
• [Presentation] Toward measurement and manipulation of multicellular circuit dynamics (2022)
  • Author(s): 和氣弘明
  • Organizer: 第45回分子生物学会
  • Invited
• [Presentation] 多細胞計測・操作技術の未来 (2022)
  • Author(s): 和氣弘明
  • Organizer: 次世代脳シンポジウム
  • Invited
• [Presentation] Physiological and Pathological functions of microglia (2022)
  • Author(s): 和氣弘明
  • Organizer: 第51回日本免疫学会学術集会
• [Presentation] 先天性筋無力症候群アップデートと神経筋接合部の構築を誘導する新規分泌因子 (2022)
  • Author(s): 大野欽司
  • Organizer: 第63回日本神経学会総会
  • Invited
• [Presentation] Three newly identified molecules that facilitate the formation of neuromuscular junction (2022)
  • Author(s): 大野欽司
  • Organizer: XIIth International Symposium on Cholinergic Mechanisms
  • Int'l Joint Research / Invited
• [Presentation] Updates on congenital myasthenic syndromes (2022)
  • Author(s): 大野欽司
  • Organizer: 20th Asian Oceanian Myology Center Meeting
  • Int'l Joint Research / Invited
• [Presentation] パーキンソン病患者腸内細菌叢の中核を担うとされるChristensenellaceae R-7の単離 (2022)
  • Author(s): 関口恭平, 大塚圭吾, 浜口知成, 大野欽司, 小林未育, 平山正昭
  • Organizer: 第63回日本神経学会総会
• [Presentation] 腸内細菌の変化はパーキンソン病の病態進行を予測できる (2022)
  • Author(s): 小関華凜, 鈴木花歩, 関口恭平, 大塚圭吾, 大野欽司, 西脇寛, 前田哲也, 坪井義夫, 柏原健一, 平山正昭
  • Organizer: 第63回日本神経学会総会
• [Presentation] RNPS1 in PSAP Complex Controls Precise Pre-mRNA Splicing with Periodicity during Cell Cycle (2022)
  • Author(s): Fukumura K, Takeda J, Masuda A, Ohno K, Mayeda A.
  • Organizer: 第23回日本RNA学会年会
• [Presentation] パーキンソン病患者の腸内細菌による分子状水素産生の低下 (2022)
  • Author(s): 西脇寛, 伊藤美佳子, 大野欽司
  • Organizer: 第11回日本分子状水素医学生物学会
• [Presentation] 分子状水素はmtUPR応答を示す細胞株の増殖を促進する (2022)
  • Author(s): 寺西正樹, 長谷川智也, 伊藤美佳子, 西脇寛, 武田淳一, Tyler LeBaron, 大野欽司
  • Organizer: 第11回日本分子状水素医学生物学会
• [Presentation] 超低周波微弱磁場は社会的敗北モデルマウスのうつ様症状を改善する (2022)
  • Author(s): 寺西正樹, 伊藤美佳子, 大野欽司
  • Organizer: 第12回名古屋大学医学系研究科・生理学研究所合同シンポジウム
• [Presentation] サルブタモールが有効だったDOK-7変異による先天性筋無力症候群の姉弟例 (2022)
  • Author(s): 小林道雄, 澤石由記夫, 大野欽司, 豊島至
  • Organizer: 第40回日本神経治療学会
• [Presentation] Painful muscle spasm with marked hyper-creatine kinase (CK) level after more than twenty weeks of four pregnant women with myotonic dystrophy type 1 (DM1) and a pregnant woman with paramyotonia congenita (PMC). (2022)
  • Author(s): Kinoshita M, Hara W, Yoshida N, Miyauchi A, Yamamoto M, Oji S, Fukaura H, Kaida K, Ohno K.
  • Organizer: 13th International Myotonic Dystrophy Consortium Meeting
  • Int'l Joint Research
• [Presentation] Novel mutations at equivalent position in E-loops of AChR and subunits result in Escobar and congenital myasthenic syndrome respectively. (2022)
  • Author(s): Shen XM, Nakata T, Mizuno S, Imoto I, Spilsbury PR, Swoboda KJ, Al-Mateen M, Selcen D, Ohno K, Engel AG.
  • Organizer: 147th American Neurology Association Annual Meeting
  • Int'l Joint Research
• [Presentation] Interactions between muscle glucose homeostasis and neuromuscular signal transduction Lessons learned from lack of a muscle-specific long isoform of GFPT1 (2022)
  • Author(s): Farshadyeganeh P, Nazim M, Ohkawara B, Masuda A, Ito M, Takeda J, Ohe K, Ohno K.
  • Organizer: European Human Genetics Conference 2022
  • Int'l Joint Research
• [Presentation] Soy protein β-conglycinin Attenuates Cardiac Hypertrophy and Heart Failure by Regulation of Gut Microbiota (2022)
  • Author(s): Furukawa N, Kondo N, Miyazaki A, Ozeki K, Koitabashi N, Nagata K, Ohno K, Hirayama M.
  • Organizer: CVMW2022 心血管代謝週間
• [Presentation] Multiple nicotinic acetylcholine receptor subtypes regulate social or cognitive behaviors in mice repeatedly administered phencyclidine (2022)
  • Author(s): Noda Y, Uchida M, Mamiya T, Yoshida M, Kitagaki S, Yoshimi A, Ozaki N.
  • Organizer: 33rd CINP Hybrid World Congress of Neuropsychopharmacology
  • Int'l Joint Research
• [Presentation] The ameliorating effect of memantine on the impairment of social behaviors induced by social defeat stress exposure as juveniles via regulating the GluN2-ERK1/2 signaling pathway (2022)
  • Author(s): Yoshida M, Suzuki C, Yoshimi A, Ozaki N, Noda Y.
  • Organizer: 33rd CINP Hybrid World Congress of Neuropsychopharmacology
  • Int'l Joint Research
• [Presentation] 新生仔期プロスタグランジンE2投与による若年・成体期の情動性・情報処理機能と遺伝子発現に及ぼす影響 (2022)
  • Author(s): 肥田裕丈, 吉田樹生, 見陽, 堀田彰悟, 山田清文, 尾崎紀夫, 野田幸裕
  • Organizer: 第141回日本薬理学会近畿部会
• [Presentation] 誘導性ニューロン移動関連分子アストロタクチン2（ASTN2）の欠失が高次脳機能と神経構築に与える影響 (2022)
  • Author(s): 吉田樹生, 伊藤貴博, 平松愉加, 相田知海, 久島周, 見陽,田中光一, 尾崎紀夫, 野田幸裕
  • Organizer: BPCNPNPPP4学会合同年会
• [Presentation] Involvement of prostaglandin E2 in psychobehavioral and neuronal impairments induced by perinatal exposure to environmental risk factor of psychiatric disorders (2022)
  • Author(s): Hida H, Mouri A, Hotta S, Uchida M, Furuyashiki T, Narumiya S, Yoshimi A, Nabeshima T, Ozaki N, Yamada K, Noda Y．
  • Organizer: Society for Neuroscience 2022
  • Int'l Joint Research
• [Presentation] Exposure to social defeat stress as juveniles leads to activated brain immune systems and impaired neuronal morphology (2022)
  • Author(s): Yoshida M, Suzuki C, Hamada M, Katada H, Hida H, Mouri A, Ozaki N, Noda Y.
  • Organizer: 第96回日本薬理学会年会/第43回日本臨床薬理学会学術総会（Japan Basic and Clinical Pharmacology Week：JPW2022）
• [Presentation] 質量分析を介した複合生命情報へのBioinformaticsによるアプローチ (2022)
  • Author(s): 財津桂
  • Organizer: 第47回日本医用マススペクトル学会年会
• [Presentation] 質量分析から得られる多変量情報の解析プラットフォームとデータ駆動型解析の有用性 (2022)
  • Author(s): 財津桂
  • Organizer: 第48回BMSコンファレンス
• [Book] Clinical Neuroscience41巻2月号 (2023)
  • Author(s): 大野欽司
  • Total Pages: 144
  • Publisher: 中外医学社
• [Book] Clinical Neuroscience40巻12月号 (2022)
  • Author(s): 進藤麻理子, 辻貴宏, 和氣弘明
  • Total Pages: 144
  • Publisher: 中外医学社
• [Book] 薬学雑誌ファルマシア58巻9号 (2022)
  • Author(s): 橋本明香里, 和氣弘明
  • Total Pages: 79
  • Publisher: 公益社団法人 日本薬学会
• [Book] 炎症と免疫vol.30no.3 (2022)
  • Author(s): 渋下碧, 竹田育子,和氣弘明
  • Total Pages: 92
  • Publisher: 先端医学社
• [Book] 実験医学2022年11月号 (2022)
  • Author(s): 和氣弘明
  • Total Pages: 121
  • Publisher: 羊土社
  • ISBN: 978-4-7581-2561-1
• [Book] Medical Science Digest2022年12月号 (2022)
  • Author(s): 和氣弘明
  • Total Pages: 56
  • Publisher: 北隆館
• [Book] BMC part of Springer Nature (2022)
  • Author(s): 和氣弘明
  • Total Pages: -
  • Publisher: BioMed Central
• [Book] 日本薬理学雑誌 特集 (2022)
  • Author(s): 和氣弘明
  • Total Pages: -
  • Publisher: 公益社団法人 日本薬理学会
• [Book] 医学のあゆみ283巻10号 (2022)
  • Author(s): 大野欽司
  • Total Pages: 120
  • Publisher: 医歯薬出版
• [Patent(Industrial Property Rights)] パーキンソン病の判定マーカーおよび判定方法 (2021)
  • Inventor(s): 辻浩和,朝原崇,野本康二,平山正昭,大野欽司
  • Industrial Property Rights Holder: 東海国立大学機構
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 7209930
  • Overseas