2023 Fiscal Year Final Research Report
Development of oxygen-supplying method for ultra-high density cell culture
Project/Area Number |
21K04800
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 27040:Biofunction and bioprocess engineering-related
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Research Institution | Osaka Institute of Technology |
Principal Investigator |
NAGAMORI Eiji 大阪工業大学, 工学部, 教授 (70394898)
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Project Period (FY) |
2021-04-01 – 2024-03-31
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Keywords | 培養工学 / 溶存酸素 / 非侵襲計測 / 組織培養 |
Outline of Final Research Achievements |
To reduce the cost of products made from suspension cell cultures, it is important to develop fundamental technologies that enable high-density culture. The realization of technology for culturing highly concentrated cellular tissues is also required. One of the bottlenecks in both of these areas is the development of technology to supply cultured cells with oxygen without shortage. In this research, as an approach to increase the rate of oxygen supply to the liquid, we aimed to utilize artificial oxygen carriers, etc., and first worked to realize high-density culture conditions in tissue culture and suspension culture in which the occurrence of oxygen rate-limiting events can be detected. Currently, we are evaluating the effects of oxygen carrier samples using the constructed experimental system with collaborators.
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Free Research Field |
生物化学工学
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Academic Significance and Societal Importance of the Research Achievements |
低酸素状態でGFP発現すMOI30#8株を用い、平面培養(2D)において画像情報から実溶存酸素濃度を推定できる可能性を示した。この技術を活用して三次元組織(3D)内の実溶存酸素濃度を非侵襲的に簡便に明らかにする技術が初めて構築された。この技術は将来の組織培養技術開発において基盤的な評価技術となり得る。 無血清培地で懸濁培養が可能なCHO細胞の高濃度培養技術について検討する中で、気泡‐細胞懸濁液分離技術を、半導体加工技術で作製された微細孔ステンレスメッシュを活用して構築した。通気培養において、気泡と細胞懸濁液を簡便に分離する技術は他用途への展開が期待できる。
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