Investigation for the mexchanism underlying accumulation of amino acids in yeast vacuoles

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K05383
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 38030:Applied biochemistry-related
• Research Institution: Ehime University
• Principal Investigator: KAWANO-KAWADA Miyuki 愛媛大学, 農学研究科, 准教授 (10454498)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: 酵母 / 液胞 / アミノ酸 / トランスポーター

Outline of Final Research Achievements

In this research, we identified SPAC24H6.11c (vsb1+), which is essential for the accumulation of basic amino acids into vacuoles of Schizosaccharomyces pombe. We also found a conserved acidic residue in the first putative transmembrane domain, Asp174, is required for its activity. Vsb1 homologs are highly conserved among fungi, suggesting that the accumulation of basic amino acids in vacuoles by Vsb1 plays an important physiological role in these organisms.

Free Research Field

生化学　分子生物学

Academic Significance and Societal Importance of the Research Achievements

本研究とこれまでの研究により、酵母液胞への塩基性アミノ酸取り込み／排出に関わる液胞膜タンパク質がほぼ明らかとなった。本研究では液胞アミノ酸トランスポーターが細胞内の栄養応答シグナル伝達に影響を与えることを示唆する結果も得られており、液胞の新たな生理機能解明に向けた足がかりとなると考えられる。