Elucidation of the nuclear receptor PPARgamma activator contained in sugarcane juice

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K05492
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 38050:Food sciences-related
• Research Institution: Okayama University of Science
• Principal Investigator: Matsuura Nobuyasu 岡山理科大学, 生命科学部, 教授 (60281250)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: サトウキビ搾汁液 / PPARγ

Outline of Final Research Achievements

We attempted to isolate and purify the nuclear receptor PPARγ activator contained in sugarcane juice. For purification, separation conditions were established by optimizing separation conditions for synthetic adsorption resins, reversed-phase chromatography, silica gel chromatography, etc. As a result, they succeeded in obtaining 20mg of active substance from 100L of sugarcane juice. After measuring the MS spectrum and nuclear magnetic resonance spectrum of the obtained substance and examining the data in detail, they succeeded in narrowing down the structure to several structural formulas. Furthermore, by analyzing the MS spectrum fragments of the chemically converted compounds, we succeeded in determining the chemical structure of the active substance.

Free Research Field

生物有機化学

Academic Significance and Societal Importance of the Research Achievements

砂糖原料であるサトウキビ搾汁液より、糖代謝に重要な役割を果たすPPARγ活性化物質を発見したことは、単に生活習慣病予防及び治療に関わる有用の知見のみならず、植物生理学の観点からも有用な知見になると考えられた。