Studies on the molecular mechanism of carbon dioxide anaethesia

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K05725
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 40030:Aquatic bioproduction science-related
• Research Institution: Kanazawa University
• Principal Investigator: Matsubara Hajime 金沢大学, 生命理工学系, 教授 (50459715)
• Co-Investigator(Kenkyū-buntansha): 鈴木 信雄 金沢大学, 環日本海域環境研究センター, 教授 (60242476)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: 魚類麻酔 / 炭酸 / ストレス関連遺伝子 / 可視化 / 魚類福祉

Outline of Final Research Achievements

The 3Rs Principle in Animal Experimentation is becoming popular in fish as well.　However, it is unknown which of the conventional fish anesthetics reduce stress in fish. So far, we have successfully visualized stress in flounder and other species. Therefore, we immersed flatfish in the conventional fish anesthetics, and compared the body color and stress-related genes for each anesthetics. In carbonic anesthesia, it was presumed that the flatfish would be anesthetized prior to the stress response. Specifically, carbonic acid was considered to have the potential to reduce stress the most among the conventional fish anesthetics.

Free Research Field

水圏生産科学

Academic Significance and Societal Importance of the Research Achievements

魚類でも麻酔薬が半世紀以上前から使用されている。しかし、魚類麻酔の薬理・生理作用の知見は少なく、魚類福祉は他の動物福祉より20年遅いとされる。本研究では、ストレスを可視化できるヒラメをもちいて、炭酸が分子レベルからも最もストレス関連因子を軽減させることがわかった。今後、炭酸の有効性をさらに調べるとともに、他の魚類のストレス軽減麻酔薬も探索し、魚類福祉の進展に寄与したい。