2023 Fiscal Year Final Research Report
Analysis of fatty acid-related signals contributing to the pathogenesis of bovine abomasum displacement and their application to early diagnostic markers
| Project/Area Number |
21K05946
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 42020:Veterinary medical science-related
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| Research Institution | Nippon Veterinary and Life Science University |
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Principal Investigator |
Tajima Tsuyoshi 日本獣医生命科学大学, 獣医学部, 講師 (60508878)
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| Co-Investigator(Kenkyū-buntansha) |
三浦 亮太朗 日本獣医生命科学大学, 獣医学部, 准教授 (60782133)
金田 剛治 日本獣医生命科学大学, 獣医学部, 教授 (10350175)
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Keywords | 牛 / 第四胃変位 / n-3脂肪酸 / n-3/AA比 / TLR4シグナル |
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| Outline of Final Research Achievements |
Blood samples were obtained from Holstein cows one week before calving and at 0-8 weeks after calving. The samples were analyzed to assess the blood fatty acid profile. A health check of the cows was also conducted to explore the relationship between abomasal displacement (DA) and ketosis, a known underlying pathological process of DA. The study investigated the correlation between DA and ketosis, which is considered the underlying factor in the development of DA.
The blood fatty acids level, including lauric acid, palmitic acid, and myristic acid, which act as endogenous toll-like receptor 4 (TLR4) activators, did not show significant differences between healthy cows and ketosis cows. However, blood levels of n-3 fatty acids (docosahexaenoic acid and docosapentaenoic acid) ratio vs arachidonic acid, which have a role in suppressing TLR4 signalling, decreased in ketosis cows within 1-2 weeks after calving.
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| Free Research Field |
獣医学
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| Academic Significance and Societal Importance of the Research Achievements |
第四胃平滑筋を用いたin vitro試験からTLR4シグナルの増強に関連する脂肪酸の分娩前後における血中動態について解析した。内因性TLR4リガンドとして働きうると推察されたラウリン酸、パルミチン酸、ミリスチン酸の血中濃度は健康牛・罹患牛ともに分娩前後で有意な変化はみられなかった。一方、n-3脂肪酸/アラキドン酸比が低下するとTLR4シグナル抑制機構の減弱に関与することから、DA発症に重要なTLRシグナル亢進の基盤として分娩直後にn-3脂肪酸が減少していることが寄与していると考えられた。
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