2023 Fiscal Year Final Research Report
N-end rule pathway of protein degradation in early development.
| Project/Area Number |
21K05979
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 42030:Animal life science-related
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| Research Institution | Kindai University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
安齋 政幸 近畿大学, 先端技術総合研究所, 教授 (30454630)
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Keywords | N末端則経路 / アルギニル化 / 初期胚 |
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| Outline of Final Research Achievements |
In this study, arginylated proteins were detected in mouse oocytes and preimplantation embryos by LC-MS/MS. Eleven of them were identified as the candidates of the targets of the proteolysis via N-end rule passwey. However, we were not able to do future analyses on the behavior or degradation of those proteins.
We were not able to analyze the embryo derived from ATE1-depleted oocytes, because we failed to obtain ATE1-depleted oocytes from ATE1-CKO mice.
The results obtained are not enough to achieve the purpose of this study. However, one of our results suggests that ATE1 is essential for female gametogenesis, providing interesting direction for the future study.
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| Free Research Field |
細胞生物学、発生生物学、生殖生物学
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| Academic Significance and Societal Importance of the Research Achievements |
受精直後の胚は卵細胞質に蓄積された母性タンパク質を利用して発生を進めるが、それと同時に母性タンパク質を分解することも必要である。N末端則経路を介したタンパク質の分解はこれまで初期胚では調べられておらず、本研究においてそのターゲットの候補となるタンパク質を検出できたことは、初期発生におけるタンパク質分解メカニズムの研究の発展につながるものであると考える。また、本研究では、ATE1が雌性配偶子形成に必須である可能性を提示した。これらの成果は、配偶子や初期胚の今後の研究に貢献できる可能性があるほか、生殖医療や家畜生産の現場での応用につながる可能性がある。
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