2023 Fiscal Year Final Research Report
Imaging analysis of ER-mitochondria contact sites in living cells
| Project/Area Number |
21K06149
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 44010:Cell biology-related
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Keywords | オルガネラ接触領域 / ダイナミクス / 可視化解析 / ミトコンドリア / 小胞体 |
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| Outline of Final Research Achievements |
Endoplasmic reticulum-mitochondria contact site (EMCS) plays an important role in various cellular activities including calcium ion transport and lipid transport, but its dynamics in living cells have remained unclear. In this study, we performed live imaging of EMCSs using a probe that utilizes a dimeric fluorescent protein, and analyzed the formation/disappearance and motility of them in living cells. The contact sites had dynamic properties even in steady state, but when cells received ligand stimulation or mechanical stimulation, we found significant increase in number and size of EMCSs, and calcium ion transfer to the mitochondria was found to be enhanced at EMCSs. Therefore, it was suggested that the function of EMCSs could be regulated by their morphological modification.
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| Free Research Field |
細胞生物学
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| Academic Significance and Societal Importance of the Research Achievements |
小胞体-ミトコンドリア接触領域は、カルシウムイオンや脂質など情報や物質をやり取りする場であり、細胞機能の維持に重要な役割をはたしている。この接触領域の機能が損なわれることにより疾病が誘導される可能性も考えられている。すなわち、それぞれのオルガネラ機能異常に加え、接触領域の機能の欠落や変調が疾病の発症基盤になると考えられる。本研究によって、接触領域の動態が接触領域の機能発現に重要な役割を果たしていることが示されたので、したがって接触領域の動態を制御することにより疾病の予防・治療方法の開発へつながる可能性が示唆された。
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