Research of signal transduction mechanism by sole LRR-type receptor in primitive red algae

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K06226
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 44030:Plant molecular biology and physiology-related
• Research Institution: The University of Tokyo
• Principal Investigator: Okuda Satohiro 東京大学, 大学院理学系研究科(理学部), 助教 (90727702)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: 受容体 / シグナル伝達 / シゾン / カイコ

Outline of Final Research Achievements

The expression and purification methods of the sole transmembrane receptor in primitive red algae Cyanidioschyzon merolae has been developed. I obtained the proteins of sufficient quality and quantity for protein crystallization screening and protein-protein interaction analysis with baculovirus-insect cell expression system. In addition, we used baculovirus-silkworm protein expression system and successfully obtained the proteins that were difficult to express in the insect cell system. These results indicate that the protein expression system using silkworm and insect cells is useful for 3D structural analysis and protein-protein interaction assays in plant research.

Free Research Field

植物分子生理学

Academic Significance and Societal Importance of the Research Achievements

植物タンパク質研究において、試験管内で生化学的解析をおこなうためには、質的、量的に安定したタンパク質発現・精製系を確立することは重要である。本研究では、昆虫培養細胞-バキュロウィルス系、カイコ幼虫-バキュロウィルス系を用いることで、タンパク質立体構造解析やタンパク質間相互作用解析に使用可能な品質のタンパク質を安定して得る方法を確立した。