2023 Fiscal Year Final Research Report
Early-life stress experiences alter adult behavior: the lateral habenula and anxiety and depression.
| Project/Area Number |
21K06371
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 46010:Neuroscience-general-related
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| Research Institution | University of Toyama |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
中村 友也 富山大学, 学術研究部医学系, 助教 (70733343)
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Keywords | 外側手綱核 / Parvalbumin / 臨界期 / 幼少期ストレス |
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| Outline of Final Research Achievements |
To investigate the vulnerability of juvenile LHb, we generated mice in which PV mRNA-transcribed cells were labeled by EGFP and examined the dynamics of PV expression. PV expression increased its transcription and translation towards P16; transcription decreased after P23, but PV-protein expression was maintained at P60. The expression history of PV showed a transcriptional and translational maximum at P16 and a transcriptional decrease from P23 onwards, consistent with the stages of the experience-dependent alteration of adult behaviors, suggesting that PV expression is involved in LHb maturation.
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| Free Research Field |
神経科学
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| Academic Significance and Societal Importance of the Research Achievements |
光遺伝学的な神経回路機能の検討は広く行われている．本研究ではPV mRNAを転写した細胞をEGFPによって標識するPV-EGFPマウスを作製することで，PV発現の履歴を明らかにした．PVの発現は動的で，PV-EGFP陽性細胞の中にはPV-proteinの発現を止めた細胞が存在した．PV-creを利用した実験では，PV-protein陰性の細胞を操作するおそれがあり，PVの転写(PV-mRNA)，翻訳(PV-protein)と履歴(PV-EGFP)からPV neuronの発現の動態を考慮した適切な実験の必要性を示した学術的影響は大きい．本研究は適切な方法を利用した研究を促進する社会的意義がある．
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