Development of novel methods for the analysis of physiological functions of modified nucleic acids and amino acids based on covalent bond formation

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K06464
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 47010:Pharmaceutical chemistry and drug development sciences-related
• Research Institution: Osaka Medical and Pharmaceutical University
• Principal Investigator: Hirano Tomoya 大阪医科薬科大学, 薬学部, 教授 (20396980)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: エピジェネティクス / エピトランスクリプトーム / 蛍光 / 生体内有機化学反応

Outline of Final Research Achievements

In living organisms, amino acids and nucleobases could be chemically modified such as methylation, and those chemical modifications could not only regulate physiological functions, but also be incriminated as the cause of several diseases. So those selective and sensitive detection methods are extremely useful. In this research, novel methods for methylation of adenine nucleotide and monomethylation of lysine residue, respectively, based on chemical reactions have been developed.

Free Research Field

ケミカルバイオロジー

Academic Significance and Societal Importance of the Research Achievements

生体内のアミノ酸、核酸塩基に対する化学修飾を選択的に検出する手法は基礎研究の発展を通じた学術的な意義だけでなく、新たな治療法の構築などによる社会的な意義も極めて大きいです。本研究では有機化学反応を利用するという独自の戦略に基づき、安価かつ簡便な検出法の開発に成功しました。開発した方法は医療、創薬などで有用となる重要な研究成果です。