Development of high-throughput glycan analysis system for understanding relationship between cellular metabolism and glycan biosynthesis

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K06505
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 47020:Pharmaceutical analytical chemistry and physicochemistry-related
• Research Institution: Kindai University
• Principal Investigator: Kinoshita Mitsuhiro 近畿大学, 薬学部, 教授 (40330279)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: マイクロチップ電気泳動 / 高スループット / 糖タンパク質糖鎖 / 代謝

Outline of Final Research Achievements

We developed a high-throughput glycan analysis system using a fully automatic microchip electrophoresis device, and applied the developed system to elucidate the relationship between metabolic fluctuations and glycan structure in a metabolic model using cells. The developed system was able to achieve a throughput improvement of more than 10 times compared to conventional glycan analysis. Furthermore, we applied this method to the analysis of metabolic changes caused by cell growth factors, etc., and revealed that sugar chains can change under the influence of metabolic changes that do not involve gene expression.

Free Research Field

分析化学

Academic Significance and Societal Importance of the Research Achievements

本研究は、マイクロチップ電気泳動装置を糖鎖解析技術として利用しつつ、糖鎖と代謝の関係に踏み込み、加齢や老化に伴う代謝の変動こそ糖鎖構造に影響を与えうる要因であることを明らかにし、同時に糖鎖研究におけるマイクロチップ電気泳動法の有用性を実証する試金石となる研究として、社会的意義は大きいと言える。