2023 Fiscal Year Final Research Report
Enhancement of MALDI ion production based on designing waveform of laser electric field and high voltage pulses
| Project/Area Number |
21K06521
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 47020:Pharmaceutical analytical chemistry and physicochemistry-related
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| Research Institution | Niigata University of Pharmacy and Medical and Life Sciences |
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Principal Investigator |
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Keywords | MALDI / 質量分析 / イオン再結合 / パルス引き出し / 数値シミュレーション / DHB / CHCA / アスパラギン酸 |
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| Outline of Final Research Achievements |
The effect of the extraction field on the ion signal intensity in the MALDI method was investigated. It was found that both positive and negative ions decreased with time in a first-order response to the delay time of the extraction electric field. This is considered to be due to the formation of pairs of positive and negative ions in close proximity in the MALDI crystal by laser irradiation, and their neutralization by recombination. Next, when the magnitude of the extraction electric field was varied, the signal intensity increased with the increase in the magnitude of the electric field. The relationship was linear in both log-log plots. This relationship between the electric field and the ion signal was successfully reproduced by constructing a model. Information on the distribution of the distance between the ion pairs generated was also obtained.
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| Free Research Field |
物理化学
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| Academic Significance and Societal Importance of the Research Achievements |
MLADI法は，熱分解しやすい生体分子などを非破壊的に気化し，高感度かつ簡便に質量分析できる方法である．定性分析としてはすぐれている一方，定量性についての評価は容易ではない．それは，MALDIにおけるイオン信号の生成過程が定量的に記述できないからである．本研究では．その中の１つの因子である，生成した正イオンと負イオンの再結合による失活に着目した．MLADIでは，レーザー照射時に生成したイオンの少なくない量が，この再結合により観測される前に消滅していると考えられる．本研究で，再結合がパルス電場の印加デザインを工夫することにより抑制できることが，定量的に示された．今後の高感度化に役立つ知見である．
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