Construction of Multiple Sample Analysis System Using FRET System and Search for Inhibitors of Virus Polymerization Proteins

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K06872
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 49010:Pathological biochemistry-related
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: Kami Daisuke 京都府立医科大学, 医学(系)研究科(研究院), 特任講師 (80415588)
• Co-Investigator(Kenkyū-buntansha): 秋光 信佳 東京大学, アイソトープ総合センター, 教授 (40294962) ; 五條 理志 京都府立医科大学, 医学(系)研究科(研究院), 教授 (90316745)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: RNA metabolism / FAPS / ウイルス由来重合タンパク質 / Cellular transition / 多検体解析

Outline of Final Research Achievements

The SARS-CoV-2 RNA virus has caused a global pandemic and competition to develop therapeutics has intensified. Most therapeutic drug targets are viral surface antigens or RNA polymerases, and there are no reports of therapeutic drug development aimed at eliminating increased viral genomic RNA in cells. Genomic RNA is stabilized intracellularly by binding to nucleocapsid protein (N-protein) and further polymerization between N-proteins. Polymerization of N-protein is inhibited by inhibition of viral synthesis, and inhibition of N-protein polymerization is also inhibited by inhibition of viral synthesis. These results and the construction of an analytical system may be a pioneering method for the development of new therapeutic agents against many viruses with N-proteins.

Free Research Field

RNA metabolism

Academic Significance and Societal Importance of the Research Achievements

RNAウイルスSARS-CoV-2の流行は世の中に対して感染症の恐ろしさと一般社会生活の脆さを示し、世界に大きな変革を与えた。この研究は感染症に対する対抗策としてRNAワクチンとは異なる手法を模索する上で重要な役割を果たしており、今後、新たな感染症が発生した場合に対抗手段として検討に値する研究成果である。また、健康寿命の延長という意味でも感染症対策は今後も重要である。