• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2023 Fiscal Year Final Research Report

Functional differentiation of anaerobic bacterial biofilms by post-transcriptional regulation

Research Project

  • PDF
Project/Area Number 21K07018
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 49050:Bacteriology-related
Research InstitutionUniversity of Tsukuba

Principal Investigator

Obana Nozomu  筑波大学, 医学医療系, 助教 (00722688)

Project Period (FY) 2021-04-01 – 2024-03-31
Keywordsバイオフィルム / 不均一性 / 偏性嫌気性細菌 / ウェルシュ菌 / 転写後制御
Outline of Final Research Achievements

This study aimed to understand the mechanisms of environmental adaptation through post-transcriptional regulation in anaerobic bacterial biofilms. We found that in response to temperature (37°C in the host to 25°C in the environment), Clostridium perfringens regulate the expression of extracellular matrix genes through transcriptional read-through regulation, resulting in changes in biofilm morphology. Furthermore, we found that gene expression involved in utilizing host components, such as sialidase genes, was up-regulated by stabilization of mRNA in response to 25°C. C. perfringens could survive by recognizing temperature and rapidly adapting to each environment by regulating biofilm morphology and nutrient availability through post-transcriptional regulation.

Free Research Field

分子微生物学

Academic Significance and Societal Importance of the Research Achievements

偏性嫌気性細菌は我々ヒトと深く関与している。一部の偏性嫌気性細菌は食中毒および院内感染や慢性感染を引き起こす起因菌(ウェルシュ菌、ディフィシル菌)であり、さらに近年腸内細菌叢はヒトの健康と深く関わることが明らかとなりつつあるが、その多くは偏性嫌気性細菌から構成される。一方で、嫌気性細菌のバイオフィルム研究例は限られており、さらに1細胞レベルの遺伝子発現・不均一性の詳細な研究例はほぼない。本申請は、これまで困難とされていた嫌気性細菌の一細胞遺伝子発現解析を実施し、その環境適応能力と病原性との関連の一端を明らかにした。本成果は医学的・基礎細菌学的観点からも大きな意義を有すると考えられる。

URL: 

Published: 2025-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi