Development of a brain environment model and pathological detection system as a basis for iPS cell analysis in Parkinson's disease

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K07301
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 51030:Pathophysiologic neuroscience-related
• Research Institution: Juntendo University
• Principal Investigator: SHIGA Takahiro 順天堂大学, 大学院医学研究科, 特任助教 (50784378)
• Co-Investigator(Kenkyū-buntansha): 野中 里紗 順天堂大学, 大学院医学研究科, 特任助教 (90614248)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: 老化 / iPS細胞

Outline of Final Research Achievements

As our country ages, there is growing concern about the increasing proportion of people suffering from late-onset neurodegenerative diseases. Although many studies have reported on neurodegenerative disease models using iPS cells, most do not consider the time axis, brain structure, or analysis techniques, resulting in few examples that replicate the pathology observed in postmortem brains. This research aimed to apply the applicant's established techniques-using low molecular compounds that promote aging and efficient induction of glial cells-to create a new device that mimics the brain structure of old age on a flat surface, and to use it for the pathological analysis of late-onset neurodegenerative diseases.

Free Research Field

神経科学

Academic Significance and Societal Importance of the Research Achievements

本研究で確立された技術は、多検体の高齢発症神経変性疾患病態解析や創薬スクリーニングに適した手法の開発を目的として構築した基盤システムであり、従来の単層培養法と比較してして高精度な結果を得ることができる。さらに、時間軸・脳内環境を模倣したモデルのため、ヒト剖検脳に近いデータを取得できる可能性を秘めている。また、様々な神経変性疾患にも応用可能なシステムなため、効率的に病態表現型や創薬スクリーニング行うことができる。