Elucidation of the relationship between changes in urinary glycans and the mechanism of IgA nephropathy

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K08230
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 53040:Nephrology-related
• Research Institution: Okayama University
• Principal Investigator: Onishi Yasuhiro 岡山大学, 医歯薬学域, 助教 (70867506)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: IgA腎症 / 糖鎖 / レクチン

Outline of Final Research Achievements

We performed a comprehensive analysis of glycans by urine lectin array analysis in a cohort of renal biopsy cases. We found three types of urine lectin binding signals (ACA, ABA, and MAH) useful as diagnostic markers for IgA nephropathy. The changes in these lectin-binding signals imply the decreased excretion of O-type glycans, which are products of C1GALT1, T antigen (Galβ1-3GalNAc). Lectin chromatography with ACA followed by mass spectrometry with iTRAQ-TMT labeling revealed the possibility that the plasminogen/plasmin regulatory system via O-glycosylation is involved in the pathogenesis in IgA nephropathy.

Free Research Field

腎臓内科学

Academic Significance and Societal Importance of the Research Achievements

IgA腎症におけるプラスミノーゲン/プラスミン制御系の分子機構の可能性が見出され、IgA腎症に対する新規治療法開発への足掛かりが得られた。