Study for the efect of excimer laser on melanocyte activation

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K08359
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 53050:Dermatology-related
• Research Institution: Nippon Medical School
• Principal Investigator: Funasaka Yoko 日本医科大学, 医学部, 教授 (30209150)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: エキシマレーザー / エキシマライト / DNA損傷 / 損傷DNA修復 / メラノサイト / アポトーシス

Outline of Final Research Achievements

Irradiation by excimer laser on C57BL/6J mouse induced more CPD formation in the lower portion of hair follicle compared with that by excimer light. Irradiation on HaCaT cells induced less CPD formation and less activation of caspase in case of excimer laser. Microarray analysis showed that excimer laser irradiation induced less apoptosis-related gene sets. Excimer light suppressed the expression of DNA repair related genes, however excimer laser suppressed that of these genes at a less degree, which indicates that excimer laser might repair CPDs more efficiently compared with excimer light.

Free Research Field

皮膚科学

Academic Significance and Societal Importance of the Research Achievements

同じ308nmの波長の光でありながら、エキシマレーザーとエキシマライトは異なる生物学的作用を有することがわかり、白斑に対する色素再生の優位性のメカニズムが毛包深部に効率よく到達することができるためであることを明らかにした。一方でDNA損傷修復遺伝子の発現低下はエキシマレーザーの方が少なく、皮膚深部にまで到達するものの皮膚癌誘発においてはより安全な光であることが判明し、光治療を行う上での重要な結果を示すことができた。