2023 Fiscal Year Final Research Report
Development of regenerative liver tissue with biliary drainage system by chemically liver progenitor cells
Project/Area Number |
21K08598
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 55010:General surgery and pediatric surgery-related
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Research Institution | Nagasaki University |
Principal Investigator |
Hidaka Masaaki 長崎大学, 医歯薬学総合研究科(医学系), 客員研究員 (10457541)
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Project Period (FY) |
2021-04-01 – 2024-03-31
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Keywords | 肝前駆細胞 / 胆管再生 / 肝再生 |
Outline of Final Research Achievements |
Liver disease imposes a significant medical burden due to a shortage of liver transplant donors and irreversible progression of liver disease. The aim of this study was to create hepatobiliary tissue organoids (HBOs) and cure liver disease. Hepatic progenitor cells (hCLiPs) were induced using human hepatocytes isolated from cirrhosis using three small molecule compounds. From those progenitor cells, they were successfully induced into bile ducts and mature hepatocytes, exhibited bile secretion and transport functions, and created organoids with both structures. It showed gene expression patterns in both bile ducts and hepatocytes. Functional patient-specific HBOs were also successfully produced from hCLiPs from cirrhotic liver tissue. Hepatobiliary tissue organoids with bile ducts in the tissue were established from human cirrhotic liver.
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Free Research Field |
再生医療
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Academic Significance and Societal Importance of the Research Achievements |
肝移植ドナーの不足と肝疾患の進行により、肝硬変、肝不全診療では依然として大きな医療負担を強いている。肝胆膵オルガノイド(HBO)は、試験管内ミニ臓器モデルを提供し、再生医療の発展に役立つ可能性がある。本研究により、ヒトHBOは、肝胆道系疾患の研究、創薬、個別化医療のための効率的なモデルとしての可能性が示された。
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