Exploration of novel therapeutic targets for gastric cancer using patient-derived organoids

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K08734
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 55020:Digestive surgery-related
• Research Institution: Hiroshima University
• Principal Investigator: Tanabe Kazuaki 広島大学, 医系科学研究科(保), 教授 (40379847)
• Co-Investigator(Kenkyū-buntansha): 坂本 直也 国立研究開発法人国立がん研究センター, 先端医療開発センター, ユニット長 (20571798)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: オルガノイド / 胃癌

Outline of Final Research Achievements

The objective of this study was to establish an organoid culture system in which patient-derived gastric cancer organoids generated from clinical specimens of gastric cancer can be used to analyze immune responses using patient-derived immune cells under three-dimensional culture and to explore new therapeutic targets. Within this research period, we established the overlay culture method, an organoid culture method capable of immune cell response. Furthermore, we confirmed the cytotoxicity and the inhibitory effect of concanamycin on gastric cancer organoids in contact with patient-derived NK cells under overlay culture. In the future, we will be able to develop a real-time intratumor microenvironmental immune response that is closer to the patient's in vivo response. The results were published in Heliyon.

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Free Research Field

胃癌

Academic Significance and Societal Importance of the Research Achievements

本研究成果であるオルガノイドオーバーレイ培養法は、これまで困難であったオルガノイドと免疫細胞応答を可能にした。我々は実際に、胃癌オルガノイドにNK細胞などの免疫細胞を接触させ免疫応答を確認し得た。本研究の成果により、たとえば癌オルガノイドを用いた新規免疫治療開発など様々な基礎研究に展開される可能性を有しており、われわれも引き続き研究発展を目指していく予定である。