Effects of transplantation of allogeneic multilayered fibroblast sheet on blood flow augmentation in the postoperative bronchial stump.

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K08904
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 55040:Respiratory surgery-related
• Research Institution: Yamaguchi University
• Principal Investigator: Tanaka Toshiki 山口大学, 医学部附属病院, 講師 (50457305)
• Co-Investigator(Kenkyū-buntansha): 村上 順一 山口大学, 医学部附属病院, 助教 (10725683)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: 気管支断端瘻の予防 / 線維芽細胞シート / 組織修復

Outline of Final Research Achievements

Multilayered fibroblast sheets produce high levels of substances that help with wound healing, such as VEGF, HGF, TGF-β, Angiopoietin-1, Angiopoietin-2, and MCO-1. When tested with vascular endothelial cells and HUVECs, the fluid from the sheet culture significantly promoted the formation of blood vessel structures. This suggests that transplanting these fibroblast sheets can stimulate the growth of new blood vessels. After one week of transplanting these fibroblast sheets, there was visible growth of new tissue in the lung's airways, and many blood vessels were observed. These results indicate that transplanting these fibroblast sheets can stimulate the growth of new blood vessels and improve blood flow, which can help avoid complications like ischemia that lead to the development of bronchial fistulas.

Free Research Field

呼吸器外科

Academic Significance and Societal Importance of the Research Achievements

細胞シート移植によって肺切除後の気管支断端瘻を予防できれば、日本ならびに世界への普及が期待できる。気管支断端瘻治療に苦しむ患者や悩む医療者を激減させ、長期にわたる医療コストをも削減することができる。また呼吸器外科手術における気管－気管や気管支－気管支吻合における縫合不全の予防、既に発症した気管支断端瘻に対する治療への適応拡大も見込まれる。さらに申請者らが最終的に目指す積層線維芽細胞シートの他家移植が可能となれば、自己の細胞・組織採取が不要な新規バイオマテリアルとして、外科他領域における術後合併症（術後出血、腸管縫合不全、術後膵液漏、胆汁漏、髄液漏、神経損傷など）の予防への応用が期待できる。