The effect of the complementation of vascular endothelial function on outcome after intestinal ischemia and reperfusion

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K09023
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 55060:Emergency medicine-related
• Research Institution: University of the Ryukyus
• Principal Investigator: HAYASHI Misuzu 琉球大学, 医学(系)研究科(研究院), 客員研究員 (60870087)
• Co-Investigator(Kenkyū-buntansha): 神里 興太 琉球大学, 医学(系)研究科(研究院), 助教 (10554454) ; 渕上 竜也 琉球大学, 病院, 講師 (10381211) ; 垣花 学 琉球大学, 医学(系)研究科(研究院), 教授 (20274897)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: 腸管虚血再灌流障害

Outline of Final Research Achievements

The new intestinal ischemia-reperfusion injury mouse model was established in wild-type and obese and diabetic mice. Intestinal ischemia-reperfusion injury was more severe in obese and diabetic mice than in wild-type mice, with higher mortality. We evaluated postoperative small intestinal mucosa, lung, and liver tissue after ischemia-reperfusion with an ischemia time that results in 75% mortality. The expression levels of NO synthase and inflammatory cytokines, which is thought to be related to decreased intestinal blood flow, were compared in the small intestinal mucosa and remote organs, but no significant differences were found. Preoperative drinking of sodium nitrite solution did not improve survival after intestinal ischemia-reperfusion surgery in obese and diabetic mice.

Free Research Field

anesthesiology

Academic Significance and Societal Importance of the Research Achievements

肥満・糖尿病を含む代謝症候群患者は年々増加しており、社会・医療経済に与える影響は大きい。腸管虚血再灌流障害は、消化管手術や心血管手術後に発生するが、肥満糖尿病マウスは野生型マウスよりも腸管虚血再灌流後の死亡率が極めて高いことが我々のマウスモデルから判明した。血管内皮機能の低下した肥満マウスにおいて亜硝酸ナトリウム水溶液の飲水が腸管虚血再灌流障害の予後を改善するか検討したが、有意な改善を認めなかった。