The Effects of myokine: irisin on fracture healing process and osteogenesis

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K09233
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 56020:Orthopedics-related
• Research Institution: Osaka Metropolitan University (2022-2023); Osaka City University (2021)
• Principal Investigator: Ohta Yoichi 大阪公立大学, 大学院医学研究科, 講師 (50633484)
• Co-Investigator(Kenkyū-buntansha): 洲鎌 亮 大阪公立大学, 大学院医学研究科, 講師 (00779457)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: マイオカイン / 骨形成タンパク / 骨形成

Outline of Final Research Achievements

This study aimed to elucidate the effects of myokine irisin on osteoblast differentiation and osteogenesis via bone morphogenetic protein (BMP) signaling pathway. In vivo, BMP-2-containing polyethylene glycol was implanted in mice, and bone microstructural analysis of ectopic bone harvested after intraperitoneal administration of irisin was performed. In vitro, C2C12 cells were induced to differentiate into osteoblasts by BMP-2 treatment, and the mRNA expression levels of osteogenesis-related genes were evaluated after irisin treatment. In vivo study showed that irisin significantly increased bone mineral content and bone volume in ectopic bone in μCT analysis. In vitro study, BMP-2/irisin co-treatment group showed significantly higher mRNA expression levels of osteogenesis-related genes than BMP treatment group.

Free Research Field

整形外科学

Academic Significance and Societal Importance of the Research Achievements

本研究により、マイオカイン：irisinによるBMP-2誘導骨形成およびBMPシグナルを介した骨芽細胞分化の促進効果が認められた。骨折や転倒は疾病負荷を増加させ、それらの主因である骨粗鬆症やサルコペニアに対する治療は、超高齢社会を迎えている現在、重要課題である。さらに、骨形成や骨折修復過程では内因性のBMPシグナルの役割が重要であるため、筋組織への同化作用も示されているirisinのBMPシグナルとの相互作用の解明は骨折治癒の新たな概念や治療法の開発に繋げることが期待できる。