2023 Fiscal Year Final Research Report

Relationship between human embryonic development dynamics and temporal and spatial changes in intracellular and extracellular electrolytes

Research Project

PDF

Project/Area Number	21K09439
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Review Section	Basic Section 56040:Obstetrics and gynecology-related
Research Institution	Akita University
Principal Investigator	Kumazawa Yukiyo 秋田大学, 医学部附属病院, 准教授 (70400504)
Co-Investigator(Kenkyū-buntansha)	藤嶋明子秋田大学, 医学部附属病院, 助教 (00866460) 寺田幸弘秋田大学, 医学系研究科, 教授 (10260431) 高橋和政秋田大学, 医学部附属病院, 技術系スタッフ (60791910)
Project Period (FY)	2021-04-01 – 2024-03-31
Keywords	胚盤胞 / Naインジケーター / Kインジケーター / Na/K ATPase / サブユニット
Outline of Final Research Achievements	In this study, a dual wavelength indicator was used to attempt more precise observation of Na/K dynamics that is not affected by the uniformity of the indicator distribution. As a result, the use of a dual wavelength indicator enabled more objective analysis of electrolyte dynamics, and this was reported at the Japan Society of Obstetrics and Gynecology and other conferences that year. Preparations are currently underway for publication of the paper. In addition, very little is known about the Na/K ATPase molecule, which plays an important role in Na/K transfer, so the isoforms of the constituent subunits α and β were analyzed using q-RT-PCR, and a paper was published that year in the international journal Human Reproduction.
Free Research Field	産婦人科学
Academic Significance and Societal Importance of the Research Achievements	保険診療化された生殖補助技術であるがその医療としての効率は向上していない。胚（受精卵）培養は生殖補助技術の基盤であるがそれに使用される培養液は未だ実験的試薬として扱われており、最善の組成に関する情報はすくない。本研究での知見のような基礎情報が積み重ねられることにより、さらに適した胚培養液が開発されることが期待され、生殖補助技術の効率向上（Time to Pregnancyの短縮）により主として受療する女性のさらなる社会進出、参入がすすむことが期待される。