Research for a genuine human adipose-derived stem cells source that can ensure quality and quantity capable for regenerative medicine

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K09783
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 56070:Plastic and reconstructive surgery-related
• Research Institution: Kindai University
• Principal Investigator: Moriyama Hiroyuki 近畿大学, 薬学総合研究所, 准教授 (90581124)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: 脂肪由来間葉系幹細胞 / シングルセル解析 / インフォマティクス解析 / トランスクリプトーム解析 / 特異的マーカー / 分化誘導 / 3Dプリンティング / 組織構築

Outline of Final Research Achievements

ScRNA-seq analysis methodology was revised, and resolution of 6 clusters was mathematically resolved to higher resolution. As a result, we identified subpopulations of cells that exhibit distinctive characteristics, including differences in differentiation directionality toward specific terminal cells and secretory characteristics, such as vacuolar factors. These results are expected to provide basic knowledge that will be useful for clinical applications. In addition, we have developed an AI analysis program specialized for multinomial comparisons including highly detailed subpopulations of this analysis system. Pseudo-time analysis also revealed that at least the shrinking population strongly influences the flow (direction of differentiation or transformation) of the two lineages.

Free Research Field

幹細胞生物学

Academic Significance and Societal Importance of the Research Achievements

本研究は、これまで未同定かつ未知の真のヒト脂肪組織間葉系間質細胞を詳らかにするものである。よって、学術的にもその意義は高い。また、この幹細胞として美容医療等の臨床に応用されている細胞群は不完全なものであり、安全性に懸念も残る。加えて、形成外科治療などの効果を高めるためには、その科学的な裏付けは不可避である。以上を背景に、本研究のもつ幹細胞の探求とその過程での細胞性状の提示は、臨床への安全性および橋渡しとしての意義に富み、社会貢献の波及効果も大きいものと思われる。