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2023 Fiscal Year Final Research Report

A novel treatment for Diabetic Refractory Ulcers utilizing culture supernatant derived from multiple stem cells

Research Project

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Project/Area Number 21K09796
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 56070:Plastic and reconstructive surgery-related
Research InstitutionJichi Medical University

Principal Investigator

Mito Nanako  自治医科大学, 医学部, 助教 (40797057)

Co-Investigator(Kenkyū-buntansha) 吉村 浩太郎  自治医科大学, 医学部, 教授 (60210762)
Project Period (FY) 2021-04-01 – 2024-03-31
Keywords脂肪間質幹細胞 / 血管内皮前駆細胞 / 培養上清 / 創傷治癒 / 難治性潰瘍
Outline of Final Research Achievements

In the present study, we successfully detoxified and concentrated human mesenchymal stem cell-derived conditioned medium using a membrane-mediated ultrafiltration method. The detoxified and concentrated conditioned medium (dt-CCM), characterized in enriched cytokines and growth factors, but less in metabolite secreted by the mesenchymal stem cells, is a potential therapeutic tool in regenerative medicine. In vitro experiments demonstrated that dt-CCM promoted the cell proliferation, cell motility, and the tube-formation of the vascular endothelial cells. Moreover, application of dt-CCM promoted the wound healing in refractory ulcer mice.

Free Research Field

形成外科学

Academic Significance and Societal Importance of the Research Achievements

糖尿病性潰瘍は、血行障害、神経障害、感染症による破壊性病変とされ、糖尿病の重大な合併症の一つである。糖尿病患者数の増加に伴い発症も増加しており、糖尿病性虚血下肢において壊死が進行すると、壊死組織の上流部を切断する治療選択を避けられず、20秒に1人の患者が下肢を切断されている(Lancet, 2008)。糖尿病性虚血下肢の治療は難渋し、効果的な治癒法の開発が期待されている分野である。本研究では、糖尿病性難治性潰瘍の治療ツールとなりうる浄化濃縮幹細胞培養上清の精製法を確立、治療効果の検証を実施した。

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Published: 2025-01-30  

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