The roles of spcific differentiation and cellular senescence in oncogenesis of odontogenic epithelium within the intraosseous microenvironment

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K09852
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 57020:Oral pathobiological science-related
• Research Institution: Tohoku University
• Principal Investigator: Kumamoto Hiroyuki 東北大学, 歯学研究科, 教授 (70215028)
• Co-Investigator(Kenkyū-buntansha): 齋藤 悠 東北大学, 歯学研究科, 助教 (00824450)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: 歯原性腫瘍 / 腫瘍発生 / 骨内進展

Outline of Final Research Achievements

Regulator molecules associated with cell cycle, epithelialcellstemness, and cellular senescence were examined to clarify the role of cell status in oncogenesis of odontogenic epithelium under the intraosseous microenvironment. Cell cycle-related molecules, Cdt1, geminin, and γ-H2A.X, were greater in ameloblastoma than in tooth germ, and Cdt1 in many neoplastic cells, geminin in peripheral neoplastic cells, and γ-H2A.X in central neoplastic cells are redognized. Epithelial stem cell markers, LGR5 and 6, and a cellular senescence-related molecule, SIRT1, were expressed higher in ameloblastoma than in tooth germ. LGR6 showed greater neoplastic cells reactivity than LGR5. SIRT1 tended to show higher expression in older patients.

Free Research Field

医歯薬学

Academic Significance and Societal Importance of the Research Achievements

正常および腫瘍性の歯原性組織における細胞周期関連分子、上皮幹細胞関連分子、細胞老化関連分子を検索し、腫瘍化による歯原性上皮細胞の特異的な状況や特徴的な腫瘍組織所見・臨床病態との関連を見出した。これらによって、歯原性腫瘍を包括する骨内微小環境下での歯原性上皮の増殖や分化に関わる歯原性上皮の最奥動態・活性状況について、検討することができた。これらの因子は、歯原性上皮が関わる様々な病変の診断や予後判定、治療に応用しうると考えられる。