Development of pulp regeneration for root canal treatment with dentin coating agent

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K09968
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 57040:Regenerative dentistry and dental engineering-related
• Research Institution: National Center for Geriatrics and Gerontology
• Principal Investigator: IOHARA Koichiro 国立研究開発法人国立長寿医療研究センター, 研究所 ジェロサイエンス研究センター, 室長 (60435865)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: 歯髄再生 / 象牙質処理

Outline of Final Research Achievements

The effects of chemicals on the dentin microenvironment in infected root canal therapy were clarified, and dentin coating materials suitable for pulp regeneration were developed. Toxicity tests showed that the calcium hydroxide and phenol formulations were toxic only on the surface, while the formalin formulation penetrated the entire dentin and was highly toxic. Cell attachment tests showed similar results. In the adipocyte induced pulp cell differentiation test, dentin EDTA-extracted protein was effective for inducing pulp regeneration. These results suggest that coatings with fibronectin, collagen, laminin, or EDTA-extracted dentin protein are highly recommended. However, in vivo studies did not show statistically significant results due to the effects of reinfection.

Free Research Field

歯内治療

Academic Significance and Societal Importance of the Research Achievements

私たちはこれまで不可逆性歯髄炎に対する歯髄再生治療を開発し、臨床研究を行った。現在、この治療は特定再生医療等委員会の承認を得ることで開業医でも実施可能である。感染根管治療への適用を広げるために、根管の無菌化が必要で、水酸化カルシウム製剤を使用した治療では歯髄の再生が難しいことをあきらかにしていた。今回の結果より、水酸化カルシウム製剤とフェノール製剤は象牙質を一層削除することで歯髄再生が適した環境にできると考えられた。ホルマリン製剤は歯髄再生治療の際には使用しない方が良いと考えられた。