Elucidation of the mechanism of pain caused by tooth movement via Piezo channels

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K10168
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 57070:Developmental dentistry-related
• Research Institution: Kyushu Dental College
• Principal Investigator: Sago Misa 九州歯科大学, 歯学部, 特別研修員 (40815825)
• Co-Investigator(Kenkyū-buntansha): 森井 葵 九州歯科大学, 歯学部, 医員 (20882046) ; 小野 堅太郎 九州歯科大学, 歯学部, 教授 (40316154) ; 川元 龍夫 九州歯科大学, 歯学部, 教授 (50323704) ; 黒石 加代子 (中尾加代子) 九州歯科大学, 歯学部, 助教 (60468303) ; 水原 正博 九州歯科大学, 歯学部, 助教 (60845402) ; 郡司掛 香織 九州歯科大学, 歯学部, 講師 (90448811)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: PIEZO

Outline of Final Research Achievements

The various changes that occur in the periodontal tissue during orthodontic treatment are all caused by mechanical stimulation called orthodontic force. However, it remains unclear how mechanical stimuli are received in periodontal tissues and how they contribute to pain development. In this study, we aimed to elucidate the mechanism of pain associated with tooth movement mediated by PIEZO channels by investigating the function and expression of PIEZO channels in human periodontal ligament fibroblasts (HPdLF). PIEZO1 on the cell membranes of HPdLFs is activated by compression force and then induces ATP release via intracellular Ca2+-dependent exocytosis and ATP-permeable channels.

Free Research Field

歯科矯正学

Academic Significance and Societal Importance of the Research Achievements

本研究の成果は、ヒト歯根膜線維芽細胞におけるPIEZOチャネルの機能及び発現を明らかにしただけではなく、矯正力を想定した圧力負荷モデルを新規に作製し、圧力負荷時における歯周組織での機械刺激受容を明らかにした。これまでに報告されている調節機構を包括的に捉えることが可能となり、矯正治療時の疼痛に対する治療法の開発、ひいては口腔顔面領域での疼痛発症機序の解明に繋がる。