Comprehensive analysis of osteocyte gene expression induced by TNF-a

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K10178
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 57070:Developmental dentistry-related
• Research Institution: Tohoku University
• Principal Investigator: Ogawa Saika 東北大学, 歯学研究科, 大学院非常勤講師 (60882397)
• Co-Investigator(Kenkyū-buntansha): 溝口 到 東北大学, 歯学研究科, 教授 (20200032) ; 北浦 英樹 東北大学, 歯学研究科, 准教授 (60295087)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: 骨細胞 / TNF / 破骨細胞

Outline of Final Research Achievements

The purpose of this study is to comprehensively analyze gene expression when TNF-a acts on osteocytes and elucidate its effects on osteoclast formation. Osteocytes were isolated using a cell sorter from 5-6 day old DMP1-Topaz mice whose osteocytes have green fluorescence. When osteocytes were cultured with TNF-a and an increase in gene expression of the chemokine Cxcl10 was found by RNA sequencing. CXCL10 did not affect osteoclast formation but promoted the migration of osteoclast precursor cells. It was suggested that CXCL10, which is expressed by osteocytes due to TNF-a, increases osteoclast formation by promoting the migration of osteoclast precursor cells.

Free Research Field

矯正歯科

Academic Significance and Societal Importance of the Research Achievements

TNF-aが骨細胞に作用した際の遺伝子発現を網羅的に調べた報告はなく、特に高純度な骨細胞に対しては、報告は非常に少ない。そのため、炎症性サイトカインであるTNF-aの骨細胞に対する作用を明らかにすることは重要な研究である。また、骨細胞の骨代謝に関する未知の作用が明らかできる可能性やさらに骨代謝だけでなく他の様々な生体反応への作用を明らかにできる可能性があり重要であると考えられる。