Effect of microgravity-cultured mesenchymal stem cells on capillaries for spinal cord ischemia-reperfusion injury

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K11240
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 59010:Rehabilitation science-related
• Research Institution: Hiroshima University
• Principal Investigator: Kurose Tomoyuki 広島大学, 医系科学研究科(保), 助教 (20363054)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: 脊髄損傷 / 虚血-再潅流障害 / 間葉系幹細胞 / 毛細血管開通 / 血管内皮細胞 / アポトーシス

Outline of Final Research Achievements

A spinal cord injury model due to ischemia-reperfusion was created using male SD rats. Immediately after reperfusion, PBS or bone marrow-derived MSCs (1G-MSCs) cultured in normal gravity, or MSCs cultured in a simulated microgravity environment (MG-MSCs) were injected. The number of open capillaries bound to tomato lectin decreased, and the improvement with MSC administration was slight.
Apoptosis was induced in spinal cord-derived vascular endothelial cells collected from 5-week-old female SD rats. MSCs were added to the endothelial cell culture environment after apoptosis induction and co-cultured. Co-culture with MSCs increased the survival rate of vascular endothelial cells and decreased the apoptosis rate. Co-culture of MG-MSCs reduced the apoptosis rate more than co-culture of 1G-MSCs.

Free Research Field

再生医療

Academic Significance and Societal Importance of the Research Achievements

MSCが血管内皮細胞のアポトーシスを抑制することを示唆し，損傷部付近における血管内皮の維持に貢献することが示唆された．MG-MSCが1G-MSCよりも高い治療効果を持つことが示唆された．