2023 Fiscal Year Final Research Report
Analysis of cellular effects by the exposure to low concentration of organically bound tritiums
| Project/Area Number |
21K12234
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 63020:Radiation influence-related
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Keywords | トリチウム / 低濃度 / 持続処理 / 有機結合型トリチウム / トリチウム水 |
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| Outline of Final Research Achievements |
The number of DNA double-strand breaks (DSBs) was investigated in normal human epithelial cells continuously exposed to tritium at low concentrations. The amount of intracellular tritium correlated with the concentration in the culture media. We also found the correlation of the intracellular amount of tritium between the cells exposed to tritiated water and that of organically bound tritium. By contrast, we found the case that the number of DSBs did not correlate with the intracellular tritium concentration. In addition, intracellular localization of tritium influenced on the induction of DSBs. Our data suggest that it is required to accumulate the data of tritium effects in cells continuously exposed at low concentration to estimate the environmental tritium risk.
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| Free Research Field |
放射線生物学
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| Academic Significance and Societal Importance of the Research Achievements |
環境レベルのトリチウムリスクは、既存の高濃度短時間暴露による知見からの外挿によって推定されるが、その正確性は不明であった。本研究では従来よりも低濃度かつ持続処理による細胞影響を検討し、環境レベルに近づけた濃度での持続処理の影響を既存の知見から推定することが困難な濃度範囲があることが判明した。また、有機結合型トリチウムにおいては細胞内局在を考慮する必要があり、種類によって細胞影響が異なることも明らかにした。いずれの場合でも環境レベルのトリチウムリスク評価には科学的知見を充足させる必要性が示された。
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