2023 Fiscal Year Annual Research Report
A novel design methodology for the construction of functionalized human cell monolayer on an egg
| Project/Area Number |
21K12667
|
|---|
| Research Institution | Kindai University Technical College |
|---|
Principal Investigator |
黄 文敬 近畿大学工業高等専門学校, 総合システム工学科 制御情報コース, 准教授 (00633413)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
張 宏 東海大学, 工学部, 特定研究員 (80774257) [Withdrawn]
|
|---|
| Project Period (FY) |
2021-04-01 – 2024-03-31
|
| Keywords | CAM microbrication / Novel distance sensor |
|---|
| Outline of Annual Research Achievements |
A plastic porous membrane with a human cell monolayer is used to separate two distinct areas of a microphysiological system to clarify cell barrier functions. However, the plastic porous membrane has low transparency/porosity. Futher, the composition and mechanical properties of a plastic membrane for cell culture are different from the native membrane. Therefore, I proposed a method to remove cells on the chorioallantoic membrane (CAM) connecting to a chick embryo and use it as a membrane for cell culture.
1) I developed a novel sensing method for sensing distance between a bubble injector tip and CAM surface.
2) I developed a novel method to remove chick epithelial cells from CAM using electrically induced microbubbles without hurting the membrane structure underneath the cell monolayer.
|
