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2023 Fiscal Year Final Research Report

A novel design methodology for the construction of functionalized human cell monolayer on an egg

Research Project

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Project/Area Number 21K12667
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 90110:Biomedical engineering-related
Research InstitutionKindai University Technical College (2023)
Kyushu University (2021-2022)

Principal Investigator

Huang Wenjing  近畿大学工業高等専門学校, 総合システム工学科 制御情報コース, 准教授 (00633413)

Co-Investigator(Kenkyū-buntansha) 張 宏  東海大学, 工学部, 特定研究員 (80774257)
Project Period (FY) 2021-04-01 – 2024-03-31
Keywordsbiomembrane fabrication / Biomembrane positioning / precise cell removal
Outline of Final Research Achievements

By locally removing cells from the chorioallantoic membrane (CAM) of a chick embryo and culturing human-derived cells on the CAM, biomedical experiments can be conducted in an environment more similar to that of humans. In this study, we propose a physical method using electrically induced bubbles to locally remove the epithelial cells on the CAM. To control the forces generated by the collapse of the microbubbles, we developed a new distance sensor that precisely measures the distance from the surface of the CAM in liquid (egg white) to the tip of the injector for bubble generation. In cell removal experiments, a pulse voltage of 500V was applied to generate the bubbles. We confirmed that cells within a circular region with a diameter of approximately 350 micrometers on the CAM can be removed when the distance between the bubble generation device and the membrane is set to 200 micrometers. This study establishes a new technique for the microfabrication of biological membranes.

Free Research Field

バイオメカニクス、医工学

Academic Significance and Societal Importance of the Research Achievements

Chick chorioallantoic membrane (CAM) is used as a model for ophthalmic research. For example, during corneal surface excision, it is necessary to precisely remove the epithelial cells on the surface. I have established a method to remove cells on the CAM without damaging the underlying structures.

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Published: 2025-01-30  

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