2022 Fiscal Year Final Research Report
Development of Cell Imaging Technique by Electrical Impedance Electro-Rotation Tomography
| Project/Area Number |
21K14072
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 19010:Fluid engineering-related
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| Research Institution | Chiba University |
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Principal Investigator |
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| Project Period (FY) |
2021-04-01 – 2023-03-31
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| Keywords | 電気トモグラフィ / 誘電回転 / 細胞イメージング / 逆問題解析 |
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| Outline of Final Research Achievements |
This study has built an electrical impedance electrorotation tomography (EIeRT) system. A numerical analysis model for controlling cell rotation has been constructed by coupling the motion equations of cell rotation and fluid dynamics. Also, a microelectrode array sensor (EIeRT sensor) capable of controlling cell rotation and measuring impedance has been designed and developed. By utilizing this system, we have successfully achieved high-resolution imaging of single cells, capturing details of the cytoplasm and cell nucleus. This accomplishment represents a significant milestone in establishing the foundation for non-invasive real-time cell monitoring.
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| Free Research Field |
生体物質輸送
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| Academic Significance and Societal Importance of the Research Achievements |
本研究では、誘電回転を利用した細胞回転制御に対する数値解析モデルを構築し、新たな細胞制御技術に関する物理的な知見を提供した。また、本イメージング手法の実現により、非侵襲リアルタイム・細胞モニタリングへの応用が期待され、細胞の内部構造や細胞を構成する細胞膜・小器官の性状を可視化する細胞イメージングにより、疾患や薬物の細胞動態を解析技術として、医療・創薬分野における革新的な技術としての基盤を築いた。
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